Recently, we demonstrated that β2-microglobulin (β2M) of amyloid deposits in hemodialysis-associated amyloidosis (HAA), a serious complication leading to hemodialysis arthropathy, is modified with advanced glycation end products (AGEs) of the Maillard reaction. In the present study, to elucidate the possible involvement of AGEs-modified β2M (AGE-β2M) in the pathogenesis of HAA, we examined the effect of AGE-β2M on macrophage production of interleukin-6 (IL-6), an important cytokine for osteoclastogenesis and bone resorption. Purified AGE-β2M from long-term hemodialysis patients, but not normal β2M, stimulated synthesis and secretion of IL-6 from macrophages. Similar effects were also induced by in vitro-prepared AGE-β2M (normal β2M incubated with glucose for 60 days in vitro). These findings suggested a potential role of AGE-β2M in the pathogenesis of HAA.
|Number of pages
|Biochemical and Biophysical Research Communications
|Published - 1994 Jun 30