TY - JOUR
T1 - γ-Aminobutyric acid-induced response in acutely isolated nucleus solitarii neurons of the rat
AU - Nakagawa, T.
AU - Wakamori, M.
AU - Shirasaki, T.
AU - Nakaye, T.
AU - Akaike, N.
PY - 1991
Y1 - 1991
N2 - The γ-aminobutyric acid (GABA)-induced macroscopic Cl- current (I(Cl)) was investigated in acutely isolated nucleus tractus solitarii (NTS) neurons by a conventional patch-clamp technique combined with a rapid drug application method. The GABA- and muscimol-induced I(Cl) increased in a concentration-dependent manner. The reversal potentials were close to the Cl- equilibrium potential. Pentobarbital sodium (PB) itself elicited a current. Bicuculline (BIC), strychnine (STR), picrotoxin, benzylpenicillin (PCG), Cd2+, and Zn2+ suppressed the GABA response in a concentration-dependent manner. Both BIC and STR shifted the concentration-response curve for GABA response to the right, whereas PCG suppressed the maximum response without affecting the threshold, indicating that BIC and STR antagonized competitively and PCG noncompetitively. The inhibitory action of PCG on GABA response was in a highly voltage-dependent manner. PB shifted the concentration-response curve for GABA response to the left. The augmentatory effect of PB was voltage dependent.
AB - The γ-aminobutyric acid (GABA)-induced macroscopic Cl- current (I(Cl)) was investigated in acutely isolated nucleus tractus solitarii (NTS) neurons by a conventional patch-clamp technique combined with a rapid drug application method. The GABA- and muscimol-induced I(Cl) increased in a concentration-dependent manner. The reversal potentials were close to the Cl- equilibrium potential. Pentobarbital sodium (PB) itself elicited a current. Bicuculline (BIC), strychnine (STR), picrotoxin, benzylpenicillin (PCG), Cd2+, and Zn2+ suppressed the GABA response in a concentration-dependent manner. Both BIC and STR shifted the concentration-response curve for GABA response to the right, whereas PCG suppressed the maximum response without affecting the threshold, indicating that BIC and STR antagonized competitively and PCG noncompetitively. The inhibitory action of PCG on GABA response was in a highly voltage-dependent manner. PB shifted the concentration-response curve for GABA response to the left. The augmentatory effect of PB was voltage dependent.
KW - Chloride current
KW - Dissociated neuron
KW - Nucleus tractus solitarius
KW - Pentobarbital
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M3 - Article
C2 - 2018109
AN - SCOPUS:0025762645
SN - 0363-6143
VL - 260
SP - C745-C749
JO - American Journal of Physiology - Cell Physiology
JF - American Journal of Physiology - Cell Physiology
IS - 4 29-4
ER -