11β-Prostaglandin F2α, a bioactive metabolite catalyzed by AKR1C3, stimulates prostaglandin F receptor and induces slug expression in breast cancer

Tomomi Yoda; Kyoko Kikuchi; Yasuhiro Miki; Yoshiaki Onodera; Shuko Hata; Kiyoshi Takagi; Yasuhiro Nakamura; Hisashi Hirakawa; Takanori Ishida; Takashi Suzuki; Noriaki Ohuchi; Hironobu Sasano; Keely May McNamara

doi:10.1016/j.mce.2015.07.008

11β-Prostaglandin F2α, a bioactive metabolite catalyzed by AKR1C3, stimulates prostaglandin F receptor and induces slug expression in breast cancer

Tomomi Yoda, Kyoko Kikuchi, Yasuhiro Miki, Yoshiaki Onodera, Shuko Hata, Kiyoshi Takagi, Yasuhiro Nakamura, Hisashi Hirakawa, Takanori Ishida, Takashi Suzuki, Noriaki Ohuchi, Hironobu Sasano, Keely May McNamara

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19 Citations (Scopus)

Abstract

Prostaglandins are a group of lipid compounds involved in inflammation and cancer. We focused on PGF2α and its stereoisomer 11β-PGF2α and examined the expression and functions of their cognate receptor (FP receptor) and metabolizing enzymes (AKR1B1 and AKR1C3 respectively) in breast cancer. In immunohistochemical analysis FP receptor status associated with adverse clinical outcome only in the AKR1C3 positive cases. Therefore, we studied FP receptor-mediated functions of 11β-PGF2α using FP receptor expressed MCF-7 cell line (MCF-FP). 11β-PGF2α treatment phosphorylated ERK and CREB and induced Slug expression through FP receptor in MCF-FP, and MCF-FP cells demonstrated decreased chemosensitivity compared to parental controls. Finally, the correlation between FP receptor and Slug was also confirmed immunohistochemically in breast cancer cases. Overall these results indicated that the actions of AKR1C3 can produce FP receptor ligands whose activation results in carcinoma cell survival in breast cancer.

Original language	English
Pages (from-to)	236-247
Number of pages	12
Journal	Molecular and Cellular Endocrinology
Volume	413
DOIs	https://doi.org/10.1016/j.mce.2015.07.008
Publication status	Published - 2015 Sept 5

Keywords

11β-Prostaglandin F2α
Aldo-keto reductase family 1 member C3
Breast cancer
In vitro models
Pathology
Prostaglandin F receptor
Slug

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.mce.2015.07.008

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Yoda, T., Kikuchi, K., Miki, Y., Onodera, Y., Hata, S., Takagi, K., Nakamura, Y., Hirakawa, H., Ishida, T., Suzuki, T., Ohuchi, N., Sasano, H., & McNamara, K. M. (2015). 11β-Prostaglandin F2α, a bioactive metabolite catalyzed by AKR1C3, stimulates prostaglandin F receptor and induces slug expression in breast cancer. Molecular and Cellular Endocrinology, 413, 236-247. https://doi.org/10.1016/j.mce.2015.07.008

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title = "11β-Prostaglandin F2α, a bioactive metabolite catalyzed by AKR1C3, stimulates prostaglandin F receptor and induces slug expression in breast cancer",

abstract = "Prostaglandins are a group of lipid compounds involved in inflammation and cancer. We focused on PGF2α and its stereoisomer 11β-PGF2α and examined the expression and functions of their cognate receptor (FP receptor) and metabolizing enzymes (AKR1B1 and AKR1C3 respectively) in breast cancer. In immunohistochemical analysis FP receptor status associated with adverse clinical outcome only in the AKR1C3 positive cases. Therefore, we studied FP receptor-mediated functions of 11β-PGF2α using FP receptor expressed MCF-7 cell line (MCF-FP). 11β-PGF2α treatment phosphorylated ERK and CREB and induced Slug expression through FP receptor in MCF-FP, and MCF-FP cells demonstrated decreased chemosensitivity compared to parental controls. Finally, the correlation between FP receptor and Slug was also confirmed immunohistochemically in breast cancer cases. Overall these results indicated that the actions of AKR1C3 can produce FP receptor ligands whose activation results in carcinoma cell survival in breast cancer.",

keywords = "11β-Prostaglandin F2α, Aldo-keto reductase family 1 member C3, Breast cancer, In vitro models, Pathology, Prostaglandin F receptor, Slug",

author = "Tomomi Yoda and Kyoko Kikuchi and Yasuhiro Miki and Yoshiaki Onodera and Shuko Hata and Kiyoshi Takagi and Yasuhiro Nakamura and Hisashi Hirakawa and Takanori Ishida and Takashi Suzuki and Noriaki Ohuchi and Hironobu Sasano and McNamara, {Keely May}",

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year = "2015",

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day = "5",

doi = "10.1016/j.mce.2015.07.008",

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Yoda, T, Kikuchi, K, Miki, Y, Onodera, Y, Hata, S, Takagi, K, Nakamura, Y, Hirakawa, H, Ishida, T , Suzuki, T, Ohuchi, N, Sasano, H & McNamara, KM 2015, '11β-Prostaglandin F2α, a bioactive metabolite catalyzed by AKR1C3, stimulates prostaglandin F receptor and induces slug expression in breast cancer', Molecular and Cellular Endocrinology, vol. 413, pp. 236-247. https://doi.org/10.1016/j.mce.2015.07.008

TY - JOUR

T1 - 11β-Prostaglandin F2α, a bioactive metabolite catalyzed by AKR1C3, stimulates prostaglandin F receptor and induces slug expression in breast cancer

AU - Yoda, Tomomi

AU - Kikuchi, Kyoko

AU - Miki, Yasuhiro

AU - Onodera, Yoshiaki

AU - Hata, Shuko

AU - Takagi, Kiyoshi

AU - Nakamura, Yasuhiro

AU - Hirakawa, Hisashi

AU - Ishida, Takanori

AU - Suzuki, Takashi

AU - Ohuchi, Noriaki

AU - Sasano, Hironobu

AU - McNamara, Keely May

PY - 2015/9/5

Y1 - 2015/9/5

N2 - Prostaglandins are a group of lipid compounds involved in inflammation and cancer. We focused on PGF2α and its stereoisomer 11β-PGF2α and examined the expression and functions of their cognate receptor (FP receptor) and metabolizing enzymes (AKR1B1 and AKR1C3 respectively) in breast cancer. In immunohistochemical analysis FP receptor status associated with adverse clinical outcome only in the AKR1C3 positive cases. Therefore, we studied FP receptor-mediated functions of 11β-PGF2α using FP receptor expressed MCF-7 cell line (MCF-FP). 11β-PGF2α treatment phosphorylated ERK and CREB and induced Slug expression through FP receptor in MCF-FP, and MCF-FP cells demonstrated decreased chemosensitivity compared to parental controls. Finally, the correlation between FP receptor and Slug was also confirmed immunohistochemically in breast cancer cases. Overall these results indicated that the actions of AKR1C3 can produce FP receptor ligands whose activation results in carcinoma cell survival in breast cancer.

AB - Prostaglandins are a group of lipid compounds involved in inflammation and cancer. We focused on PGF2α and its stereoisomer 11β-PGF2α and examined the expression and functions of their cognate receptor (FP receptor) and metabolizing enzymes (AKR1B1 and AKR1C3 respectively) in breast cancer. In immunohistochemical analysis FP receptor status associated with adverse clinical outcome only in the AKR1C3 positive cases. Therefore, we studied FP receptor-mediated functions of 11β-PGF2α using FP receptor expressed MCF-7 cell line (MCF-FP). 11β-PGF2α treatment phosphorylated ERK and CREB and induced Slug expression through FP receptor in MCF-FP, and MCF-FP cells demonstrated decreased chemosensitivity compared to parental controls. Finally, the correlation between FP receptor and Slug was also confirmed immunohistochemically in breast cancer cases. Overall these results indicated that the actions of AKR1C3 can produce FP receptor ligands whose activation results in carcinoma cell survival in breast cancer.

KW - 11β-Prostaglandin F2α

KW - Aldo-keto reductase family 1 member C3

KW - Breast cancer

KW - In vitro models

KW - Pathology

KW - Prostaglandin F receptor

KW - Slug

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U2 - 10.1016/j.mce.2015.07.008

DO - 10.1016/j.mce.2015.07.008

M3 - Article

C2 - 26170067

AN - SCOPUS:84938117798

SN - 0303-7207

VL - 413

SP - 236

EP - 247

JO - Molecular and Cellular Endocrinology

JF - Molecular and Cellular Endocrinology

ER -

11β-Prostaglandin F2α, a bioactive metabolite catalyzed by AKR1C3, stimulates prostaglandin F receptor and induces slug expression in breast cancer

Abstract

Keywords

UN SDGs

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