1α25-dihydroxyvitamin D3 induces very low density lipoprotein receptor mRNA expression in HL-60 cells in association with monocytic differentiation

Mitsuyuki Kohno; Sadao Takahashi; Koji Oida; Jinya Suzuki; Toshitaka Tamai; Tokuo Yamamoto; Tsuguhiko Nakai

doi:10.1016/S0021-9150(97)00112-3

1α25-dihydroxyvitamin D₃ induces very low density lipoprotein receptor mRNA expression in HL-60 cells in association with monocytic differentiation

Mitsuyuki Kohno, Sadao Takahashi, Koji Oida, Jinya Suzuki, Toshitaka Tamai, Tokuo Yamamoto, Tsuguhiko Nakai

Division of Aging Science

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Expression of VLDL receptor mRNA during differentiation of HL-60 cells was investigated by Northern analysis. The expression induced in 1α,25-dihydroxyvitamin D₃ (1α,25(OH)₂D₃)-treated cells was 3 times that in untreated cells, while LDL receptor mRNA expression was unchanged. VLDL receptor mRNA levels were not changed in macrophages caused to differentiate from HL-60 cells by treatment with phorbol 12-myristate 13-acetate (PMA). Treatment of sarcoma cells which possess the vitamin D receptor (MG-63 cell line) with 1α,25(OH)₂D₃ did not affect VLDL receptor mRNA levels. Therefore, 1α,25(OH)₂D₃ induces VLDL receptor mRNA in HL-60 cells through differentiation-dependent mechanisms.

Original language	English
Pages (from-to)	45-49
Number of pages	5
Journal	Atherosclerosis
Volume	133
Issue number	1
DOIs	https://doi.org/10.1016/S0021-9150(97)00112-3
Publication status	Published - 1997

Keywords

1α,25-dihydroxyvitamin D
Cell differentiation
HL-60 cells
Macrophages
Monocytic cells
VLDL receptor

ASJC Scopus subject areas

Cardiology and Cardiovascular Medicine

Access to Document

10.1016/S0021-9150(97)00112-3

Cite this

@article{2b90b6a4ec5b4af79a94c1715b15cd3a,

title = "1α25-dihydroxyvitamin D3 induces very low density lipoprotein receptor mRNA expression in HL-60 cells in association with monocytic differentiation",

abstract = "Expression of VLDL receptor mRNA during differentiation of HL-60 cells was investigated by Northern analysis. The expression induced in 1α,25-dihydroxyvitamin D3 (1α,25(OH)2D3)-treated cells was 3 times that in untreated cells, while LDL receptor mRNA expression was unchanged. VLDL receptor mRNA levels were not changed in macrophages caused to differentiate from HL-60 cells by treatment with phorbol 12-myristate 13-acetate (PMA). Treatment of sarcoma cells which possess the vitamin D receptor (MG-63 cell line) with 1α,25(OH)2D3 did not affect VLDL receptor mRNA levels. Therefore, 1α,25(OH)2D3 induces VLDL receptor mRNA in HL-60 cells through differentiation-dependent mechanisms.",

keywords = "1α,25-dihydroxyvitamin D, Cell differentiation, HL-60 cells, Macrophages, Monocytic cells, VLDL receptor",

author = "Mitsuyuki Kohno and Sadao Takahashi and Koji Oida and Jinya Suzuki and Toshitaka Tamai and Tokuo Yamamoto and Tsuguhiko Nakai",

year = "1997",

doi = "10.1016/S0021-9150(97)00112-3",

language = "English",

volume = "133",

pages = "45--49",

journal = "Atherosclerosis",

issn = "0021-9150",

publisher = "Elsevier Ireland Ltd",

number = "1",

}

TY - JOUR

T1 - 1α25-dihydroxyvitamin D3 induces very low density lipoprotein receptor mRNA expression in HL-60 cells in association with monocytic differentiation

AU - Kohno, Mitsuyuki

AU - Takahashi, Sadao

AU - Oida, Koji

AU - Suzuki, Jinya

AU - Tamai, Toshitaka

AU - Yamamoto, Tokuo

AU - Nakai, Tsuguhiko

PY - 1997

Y1 - 1997

N2 - Expression of VLDL receptor mRNA during differentiation of HL-60 cells was investigated by Northern analysis. The expression induced in 1α,25-dihydroxyvitamin D3 (1α,25(OH)2D3)-treated cells was 3 times that in untreated cells, while LDL receptor mRNA expression was unchanged. VLDL receptor mRNA levels were not changed in macrophages caused to differentiate from HL-60 cells by treatment with phorbol 12-myristate 13-acetate (PMA). Treatment of sarcoma cells which possess the vitamin D receptor (MG-63 cell line) with 1α,25(OH)2D3 did not affect VLDL receptor mRNA levels. Therefore, 1α,25(OH)2D3 induces VLDL receptor mRNA in HL-60 cells through differentiation-dependent mechanisms.

AB - Expression of VLDL receptor mRNA during differentiation of HL-60 cells was investigated by Northern analysis. The expression induced in 1α,25-dihydroxyvitamin D3 (1α,25(OH)2D3)-treated cells was 3 times that in untreated cells, while LDL receptor mRNA expression was unchanged. VLDL receptor mRNA levels were not changed in macrophages caused to differentiate from HL-60 cells by treatment with phorbol 12-myristate 13-acetate (PMA). Treatment of sarcoma cells which possess the vitamin D receptor (MG-63 cell line) with 1α,25(OH)2D3 did not affect VLDL receptor mRNA levels. Therefore, 1α,25(OH)2D3 induces VLDL receptor mRNA in HL-60 cells through differentiation-dependent mechanisms.

KW - 1α,25-dihydroxyvitamin D

KW - Cell differentiation

KW - HL-60 cells

KW - Macrophages

KW - Monocytic cells

KW - VLDL receptor

UR - http://www.scopus.com/inward/record.url?scp=0030763678&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030763678&partnerID=8YFLogxK

U2 - 10.1016/S0021-9150(97)00112-3

DO - 10.1016/S0021-9150(97)00112-3

M3 - Article

C2 - 9258406

AN - SCOPUS:0030763678

SN - 0021-9150

VL - 133

SP - 45

EP - 49

JO - Atherosclerosis

JF - Atherosclerosis

IS - 1

ER -