2-aminopurine-modified abasic-site-containing duplex DNA for highly selective detection of theophylline

Minjie Li; Yusuke Sato; Seiichi Nishizawa; Takehiro Seino; Kodai Nakamura; Norio Teramae

doi:10.1021/ja8095625

2-aminopurine-modified abasic-site-containing duplex DNA for highly selective detection of theophylline

Minjie Li, Yusuke Sato, Seiichi Nishizawa, Takehiro Seino, Kodai Nakamura, Norio Teramae

SCI - Chemistry

Tohoku University

Research output: Contribution to journal › Article › peer-review

62 Citations (Scopus)

Abstract

2-Aminopurine-modified abasic-site-containing duplex [DNA 5́-TCTGC GTCCT PXT TAACG CACAC-3́/3́-AGACG CAGGA TCA ATTGC GTGTG-5́; P = 2-aminopurine, X = abasic site (Spacer-C3), C = receptor base] is capable of selectively binding to the bronchodilator theophylline with a dissociation constant of 10 μM (5 °C, pH 7.0, I = 0.11 M) and is applicable to monitoring serum theophylline concentrations.

Original language	English
Pages (from-to)	2448-2449
Number of pages	2
Journal	Journal of the American Chemical Society
Volume	131
Issue number	7
DOIs	https://doi.org/10.1021/ja8095625
Publication status	Published - 2009 Feb 25

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abstract = "2-Aminopurine-modified abasic-site-containing duplex [DNA {\'5}-TCTGC GTCCT PXT TAACG CACAC-{\'3}/{\'3}-AGACG CAGGA TCA ATTGC GTGTG-{\'5}; P = 2-aminopurine, X = abasic site (Spacer-C3), C = receptor base] is capable of selectively binding to the bronchodilator theophylline with a dissociation constant of 10 μM (5 °C, pH 7.0, I = 0.11 M) and is applicable to monitoring serum theophylline concentrations.",

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T1 - 2-aminopurine-modified abasic-site-containing duplex DNA for highly selective detection of theophylline

AU - Li, Minjie

AU - Sato, Yusuke

AU - Nishizawa, Seiichi

AU - Seino, Takehiro

AU - Nakamura, Kodai

AU - Teramae, Norio

PY - 2009/2/25

Y1 - 2009/2/25

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AB - 2-Aminopurine-modified abasic-site-containing duplex [DNA 5́-TCTGC GTCCT PXT TAACG CACAC-3́/3́-AGACG CAGGA TCA ATTGC GTGTG-5́; P = 2-aminopurine, X = abasic site (Spacer-C3), C = receptor base] is capable of selectively binding to the bronchodilator theophylline with a dissociation constant of 10 μM (5 °C, pH 7.0, I = 0.11 M) and is applicable to monitoring serum theophylline concentrations.

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2-aminopurine-modified abasic-site-containing duplex DNA for highly selective detection of theophylline

Abstract

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