TY - JOUR
T1 - 3D evaluation of the lamina cribrosa with swept-source optical coherence tomography in normal tension glaucoma
AU - Omodaka, Kazuko
AU - Horii, Takaaki
AU - Takahashi, Seri
AU - Kikawa, Tsutomu
AU - Matsumoto, Akiko
AU - Shiga, Yukihiro
AU - Maruyama, Kazuichi
AU - Yuasa, Tetsuya
AU - Akiba, Masahiro
AU - Nakazawa, Toru
N1 - Funding Information:
The authors have read the journal’s policies and noted the following conflicts: The authors disclose a financial relationship with Topcon Corporation because of the patent pending related to this article applied by Kazuko Omodaka, Tsutomu Kikawa, Akiko Matsumoto, Masahiro Akiba, and Toru Nakazawa. These works were supported by a JST grant from JSPS KAKENHI Grants-in-Aid for Scientific Research (B) (Toru Nakazawa, 26293372) and for Exploratory Research (Toru Nakazawa, 26670751), and by the JST Center for Revitalization Promotion. Tsutomu Kikawa, Akiko Matsumoto, and Masahiro Akiba are employed by Topcon Corporation and project members of grant for JST Center for Revitalization Promotion. The authors have nothing else to declare other than this, and this does not alter the authors’ adherence to PLOS ONE policies on sharing data and materials.
Publisher Copyright:
© 2015 Omodaka et al.
PY - 2015/4/15
Y1 - 2015/4/15
N2 - Purpose: Although the lamina cribrosa (LC) is the primary site of axonal damage in glaucoma, adequate methods to image and measure it are currently lacking. Here, we describe a noninvasive, in vivo method of evaluating the LC, based on swept-source optical coherence tomography (SS-OCT), and determine this method's ability to quantify LC thickness. Methods: This study comprised 54 eyes, including normal (n = 18), preperimetric glaucoma (PPG; n = 18), and normal tension glaucoma (NTG; n = 18) eyes.We used SS-OCT to obtain 3 x 3 mm cube scans of an area centered on the optic disc, and then synchronized reconstructed Band en-face images from this data. We identified the LC in these B-scan images by marking the visible borders of the LC pores. We marked points on the anterior and posterior borders of the LC in 12 B-scan images in order to create a skeleton model of the LC. Finally, we used B-spline interpolation to form a 3D model of the LC, including only reliably measured scan areas. We calculated the average LC thickness (avgLCT) in this model and used Spearman's rank correlation coefficient to compare it with circumpapillary retinal nerve fiber layer thickness (cpRNFLT). Results: We found that the correlation coefficient of avgLCT and cpRNFLT was 0.64 (p < 0.01). The coefficient of variation for avgLCT was 5.1%. AvgLCT differed significantly in the groups (normal: 282.6 ± 20.6 μm, PPG: 261.4 ± 15.8 μm, NTG: 232.6 ± 33.3 μm). The normal, PPG and NTG groups did not significantly differ in age, sex, refractive error or intraocular pressure (IOP), although the normal and NTG groups differed significantly in cpRNFLT and Humphrey field analyzer measurements of mean deviation. Conclusion: Thus, our results indicate that the parameters of our newly developed method of measuring LC thickness with SS-OCT may provide useful and important data for glaucoma diagnosis and research.
AB - Purpose: Although the lamina cribrosa (LC) is the primary site of axonal damage in glaucoma, adequate methods to image and measure it are currently lacking. Here, we describe a noninvasive, in vivo method of evaluating the LC, based on swept-source optical coherence tomography (SS-OCT), and determine this method's ability to quantify LC thickness. Methods: This study comprised 54 eyes, including normal (n = 18), preperimetric glaucoma (PPG; n = 18), and normal tension glaucoma (NTG; n = 18) eyes.We used SS-OCT to obtain 3 x 3 mm cube scans of an area centered on the optic disc, and then synchronized reconstructed Band en-face images from this data. We identified the LC in these B-scan images by marking the visible borders of the LC pores. We marked points on the anterior and posterior borders of the LC in 12 B-scan images in order to create a skeleton model of the LC. Finally, we used B-spline interpolation to form a 3D model of the LC, including only reliably measured scan areas. We calculated the average LC thickness (avgLCT) in this model and used Spearman's rank correlation coefficient to compare it with circumpapillary retinal nerve fiber layer thickness (cpRNFLT). Results: We found that the correlation coefficient of avgLCT and cpRNFLT was 0.64 (p < 0.01). The coefficient of variation for avgLCT was 5.1%. AvgLCT differed significantly in the groups (normal: 282.6 ± 20.6 μm, PPG: 261.4 ± 15.8 μm, NTG: 232.6 ± 33.3 μm). The normal, PPG and NTG groups did not significantly differ in age, sex, refractive error or intraocular pressure (IOP), although the normal and NTG groups differed significantly in cpRNFLT and Humphrey field analyzer measurements of mean deviation. Conclusion: Thus, our results indicate that the parameters of our newly developed method of measuring LC thickness with SS-OCT may provide useful and important data for glaucoma diagnosis and research.
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U2 - 10.1371/journal.pone.0122347
DO - 10.1371/journal.pone.0122347
M3 - Article
C2 - 25875096
AN - SCOPUS:84928905642
SN - 1932-6203
VL - 10
JO - PLoS ONE
JF - PLoS ONE
IS - 4
M1 - e0122347
ER -