5-aminosalicylic acid inhibits leukotriene B4ω-hydroxylase activity in human polymorphonuclear leukocytes

A. Ikehata, N. Hiwatashi, Y. Kinouchi, K. Ito, H. Yamazaki, T. Toyota

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


ω-oxidation is regarded as the major pathway for the metabolism and inactivation of leukotriene B4 (LTB4). To investigate the action of 5-aminosalicylic acid (5-ASA) on LTB4ω-hydroxylase activity, we incubated human polymorphonuclear leukocytes (PMNLs) with 3H-labeled LTB4 after pre-incubation with various concentrations of 5-ASA. ω-oxidation metabolites were separated by high performance liquid chromatography and each radioactivity was measured by a liquid scintilation counter. LTB4ω-hydroxylase activity was inhibited by 5-ASA in a concentration-dependent fashion. The 50% inhibitory dose was about 50 μmol/l, which is within the concentration range found in the colonic mucosa. Our findings may be valuable in elucidating the potentially critical aspect of 5-ASA treatment in ulcerative colitis (UC).

Original languageEnglish
Pages (from-to)317-320
Number of pages4
JournalProstaglandins Leukotrienes and Essential Fatty Acids
Issue number6
Publication statusPublished - 1994 Jun


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