@article{c6e94227e6364a9f9171474b58ab3ea2,
title = "8-Nitro-cGMP Attenuates the Interaction between SNARE Complex and Complexin through S-Guanylation of SNAP-25",
abstract = "8-Nitroguanosine 3′,5′-cyclic monophosphate (8-nitro-cGMP) is the second messenger in nitric oxide/reactive oxygen species redox signaling. This molecule covalently binds to protein thiol groups, called S-guanylation, and exerts various biological functions. Recently, we have identified synaptosomal-associated protein 25 (SNAP-25) as a target of S-guanylation, and demonstrated that S-guanylation of SNAP25 enhanced SNARE complex formation. In this study, we have examined the effects of S-guanylation of SNAP-25 on the interaction between the SNARE complex and complexin (cplx), which binds to the SNARE complex with a high affinity. Pull-down assays and coimmunoprecipitation experiments have revealed that S-guanylation of Cys90 in SNAP-25 attenuates the interaction between the SNARE complex and cplx. In addition, blue native-PAGE followed by Western blot analysis revealed that the amount of cplx detected at a high molecular weight decreased upon 8-nitro-cGMP treatment in SH-SY5Y cells. These results demonstrated for the first time that S-guanylation of SNAP-25 attenuates the interaction between the SNARE complex and cplx.",
keywords = "8-nitro-cGMP, complexin, exocytosis, S-guanylation, SNAP-25, SNARE complex",
author = "Yusuke Kishimoto and Kohei Kunieda and Atsushi Kitamura and Yuki Kakihana and Takaaki Akaike and Hideshi Ihara",
note = "Funding Information: *Mailing address: #608 Bldg. C10, 1-1 Gakuen-cho, Nakaku, Sakai, Osaka 599-8531, Japan. Tel: +81-72-254-9753. E-mail: ihara@b.s.osakafu-u.ac.jp. ORCID Takaaki Akaike: 0000-0002-0623-1710 Hideshi Ihara: 0000-0002-8971-5138 Author Contributions Y.K., K.K., T.A., and H.I. designed the research. Y.K., K.K., and A.K. performed experiments and analysis and interpreted the data. H.I. conducted the experiments. Y.K. and H.I. wrote the manuscript. Funding This work was supported in part by a Grant-in-Aid for a Grant-in-Aid for Scientific Research A (25253020 to T.A.), a Grant-in-Aid for Scientific Research B (16H04674 to H.I.), a Grant-in-Aid for Challenging Exploratory Research (16K15208 to T.A. and 16K13089 to H.I.), and a Grant-in-Aid for Scientific Research on Innovative Areas (Research in a Proposed Area) (26111008 to T.A. and 26111011 to H.I.) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan. Notes The authors declare no competing financial interest. Publisher Copyright: {\textcopyright} 2017 American Chemical Society.",
year = "2018",
month = feb,
day = "21",
doi = "10.1021/acschemneuro.7b00363",
language = "English",
volume = "9",
pages = "217--223",
journal = "ACS Chemical Neuroscience",
issn = "1948-7193",
publisher = "American Chemical Society",
number = "2",
}