TY - JOUR
T1 - 9,13-di-cis-Retinoic acid induces the production of tPA and activation of latent TGF-β via RARα in a human liver stellate cell line, LI90
AU - Imai, Shoko
AU - Okuno, Masataka
AU - Moriwaki, Hisataka
AU - Muto, Yasutoshi
AU - Murakami, Kazuhiro
AU - Shudo, Koichi
AU - Suzuki, Yasuhiro
AU - Kojima, Soichi
N1 - Funding Information:
This study was supported partly by Grant-in-Aids from the Ministry of Education, Science, Sports and Culture (05770350, M.O.; 05670463, H.M.; 08780689, S.K.), a grant from a Haraguchi Memorial Cancer Research Fund (M.O.), Grants for Biodesign Research Program and Multi-bioprobe Research Program from RIKEN (S.K.), and by grants from The Naito Foundation (S.K.).
PY - 1997/7/7
Y1 - 1997/7/7
N2 - We studied the mechanism by which 9,13-di-cis-retinoic acid (9,13dcRA), a novel and endogenous stereoisomer of all-trans-RA, induces TGF-β formation in a human liver stellate cell line, LI90. 9,13dcRA induced the expression of RARα and RARβ, enhanced the production of tissue-type plasminogen activator (tPA), thereby, surface plasmin levels, and induced the activation of latent TGF-β. Similar effects were obtained with RARα-selective retinoid, but not with RARβ- or RARγ-selective retinoid, and the induction was inhibited by RARα-selective antagonist. These results suggest that 9,13dcRA up-regulates tPA expression, resulting in the formation of TGF-β by LI90 cells, at least in part, via induction and activation of RARα.
AB - We studied the mechanism by which 9,13-di-cis-retinoic acid (9,13dcRA), a novel and endogenous stereoisomer of all-trans-RA, induces TGF-β formation in a human liver stellate cell line, LI90. 9,13dcRA induced the expression of RARα and RARβ, enhanced the production of tissue-type plasminogen activator (tPA), thereby, surface plasmin levels, and induced the activation of latent TGF-β. Similar effects were obtained with RARα-selective retinoid, but not with RARβ- or RARγ-selective retinoid, and the induction was inhibited by RARα-selective antagonist. These results suggest that 9,13dcRA up-regulates tPA expression, resulting in the formation of TGF-β by LI90 cells, at least in part, via induction and activation of RARα.
KW - 9,13-di-cis-Retinoic acid
KW - Liver stellate cell
KW - Proteolytic activation
KW - RARα
KW - TGF-β
KW - tPA
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U2 - 10.1016/S0014-5793(97)00673-X
DO - 10.1016/S0014-5793(97)00673-X
M3 - Article
C2 - 9247151
AN - SCOPUS:0030810959
SN - 0014-5793
VL - 411
SP - 102
EP - 106
JO - FEBS Letters
JF - FEBS Letters
IS - 1
ER -