TY - JOUR
T1 - A 570-kb DNA sequence of the escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map
AU - Aiba, Hiroji
AU - Baba, Tomoya
AU - Hayashi, Kouji
AU - Inada, Toshifumi
AU - Isono, Katumi
AU - Itoh, Takeshi
AU - Kasai, Hiroaki
AU - Kashimoto, Kaoru
AU - Kimura, Shigenobu
AU - Kitakawa, Madoka
AU - Kitagawa, Masanari
AU - Making, Kozo
AU - Miki, Takeyoshi
AU - Mizobuchi, Kiyoshi
AU - Mori, Hirotada
AU - Mori, Tomoko
AU - Motomura, Kouji
AU - Nakade, Shinsuke
AU - Nakamura, Yoshikazu
AU - Nashimoto, Hiroko
AU - Nishio, Yoshitaka
AU - Oshima, Taku
AU - Saito, Noriko
AU - Sampei, Gen Ichi
AU - Seki, Yasushi
AU - Sivasundaram, Suharnan
AU - Tagami, Hideaki
AU - Takeda, Jun Ichi
AU - Takemoto, Keiko
AU - Takeuchi, Yasushi
AU - Wada, Chieko
AU - Yamamoto, Yoshihiro
AU - Horiuchi, Takashi
PY - 1996
Y1 - 1996
N2 - The 569,750 base pair sequence corresponding to the 28.0-40.1 min region on the genetic map of Escherichia coli K-12 (W3110) was determined. This region includes the replication terminus region and contained at least 549 potential open reading frames. Among them, 160 (29%) were previously reported, 174 (32%) were homologous to other known genes, 102 (18%) were identical or similar to hypothetical genes registered in databases, and the remaining 113 (21%) did not show a significant similarity to any other gene. Of interest was the finding of a large number of genes and gene clusters in and near the replication termination region which had been thought to be genetically silent. Those included a cluster of genes for fatty acid β-oxidation, the third copy of the pot (spermidine/putrescine transport system) gene cluster, the second dpp (dipeptide transport system) operon, the second dsm (anaerobic dimethyl sulfoxide reductase) operon, a cluster of fim (fimbrial) genes and a DNA helicase-like gene with a high molecular weight. In addition, we found the dnaC- and dnaT-like genes in the cryptic prophage, Rac, and a number of genes originated probably from plasmids.
AB - The 569,750 base pair sequence corresponding to the 28.0-40.1 min region on the genetic map of Escherichia coli K-12 (W3110) was determined. This region includes the replication terminus region and contained at least 549 potential open reading frames. Among them, 160 (29%) were previously reported, 174 (32%) were homologous to other known genes, 102 (18%) were identical or similar to hypothetical genes registered in databases, and the remaining 113 (21%) did not show a significant similarity to any other gene. Of interest was the finding of a large number of genes and gene clusters in and near the replication termination region which had been thought to be genetically silent. Those included a cluster of genes for fatty acid β-oxidation, the third copy of the pot (spermidine/putrescine transport system) gene cluster, the second dpp (dipeptide transport system) operon, the second dsm (anaerobic dimethyl sulfoxide reductase) operon, a cluster of fim (fimbrial) genes and a DNA helicase-like gene with a high molecular weight. In addition, we found the dnaC- and dnaT-like genes in the cryptic prophage, Rac, and a number of genes originated probably from plasmids.
KW - Escherichia coli K12
KW - Genome sequence
KW - ORF analysis
KW - Replication termination region
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U2 - 10.1093/dnares/3.6.363
DO - 10.1093/dnares/3.6.363
M3 - Article
C2 - 9097039
AN - SCOPUS:0030608406
SN - 1340-2838
VL - 3
SP - 363
EP - 377
JO - DNA Research
JF - DNA Research
IS - 6
ER -