A cell cycle-dependent co-repressor mediates photoreceptor cell-specific nuclear receptor function

Shinichiro Takezawa, Atsushi Yokoyama, Maiko Okada, Ryoji Fujiki, Aya Iriyama, Yasuo Yanagi, Hiroaki Ito, Ichiro Takada, Masahiko Kishimoto, Atsushi Miyajima, Ken Ichi Takeyama, Kazuhiko Umesono, Hirochika Kitagawa, Shigeaki Kato

Research output: Contribution to journalArticlepeer-review

44 Citations (Scopus)


Photoreceptor cell-specific nuclear receptor (PNR) (NR2E3) acts as a sequence-specific repressor that controls neuronal differentiation in the developing retina. We identified a novel PNR co-repressor, Ret-CoR, that is expressed in the developing retina and brain. Biochemical purification of Ret-CoR identified a multiprotein complex that included E2F/Myb-associated proteins, histone deacetylases (HDACs) and NCoR/HDAC complex-related components. Ret-CoR appeared to function as a platform protein for the complex, and interacted with PNR via two CoRNR motifs. Purified Ret-CoR complex exhibited HDAC activity, co-repressed PNR transrepression function in vitro, and co-repressed PNR function in PNR target gene promoters, presumably in the retinal progenitor cells. Notably, the appearance of Ret-CoR protein was cell-cycle-stage-dependent (from G1 to S). Therefore, Ret-CoR appears to act as a component of an HDAC co-repressor complex that supports PNR repression function in the developing retina, and may represent a co-regulator class that supports transcriptional regulator function via cell-cycle-dependent expression.

Original languageEnglish
Pages (from-to)764-774
Number of pages11
JournalEMBO Journal
Issue number3
Publication statusPublished - 2007 Feb 7


  • Cell cycle
  • Co-repressor complex
  • HDAC
  • PNR
  • Retina


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