TY - JOUR
T1 - A comparison study in the proteomic signatures of multipotent germline stem cells, embryonic stem cells, and germline stem cells
AU - Kurosaki, Hajime
AU - Kazuki, Yasuhiro
AU - Hiratsuka, Masaharu
AU - Inoue, Toshiaki
AU - Matsui, Yasuhisa
AU - Wang, Chi Chiu
AU - Kanatsu-Shinohara, Mito
AU - Shinohara, Takashi
AU - Toda, Tosifusa
AU - Oshimura, Mitsuo
N1 - Funding Information:
We are grateful to Dr. C.L. Stewart (Roche Institute of Molecular Biology, Nutley, NJ) for providing the EG-1 and EG-3 cells and Dr. Patricia A. Lavosky (University of Pennsylvania School of Medicine) for providing the TGC8.5 and TGC12.5 cells. We also thank Mr. Hiraku Morisawa (Proteomics Collaboration Research Group, Tokyo Metropolitan Institute of Gerontology) for technical advice. This study was supported in part by a Health and Labour Sciences Research Grant for Research on Human Genome, Tissue Engineering from the Ministry of Health, Labour and Welfare, Japan; the 21st century COE program: the Research Core for Chromosome Engineering Technology, Japan; the Ministry of Education, Culture, Sports, Science and Technology, Japan; and Li Ka Shing Institute of Health Sciences Grant, The Chinese University of Hong Kong, Hong Kong.
PY - 2007/2/9
Y1 - 2007/2/9
N2 - Germline stem (GS) cells can only differentiate into germline cells, while multipotent germ stem (mGS) cells, like embryonic stem (ES) cells, can differentiate into various somatic cells and tissues. The proteomic profiles in GS and mGS cells were compared by two-dimensional gel electrophoresis. Ten down-regulated and 16 up-regulated proteins were differentially expressed in mGS cells in comparison to GS cells, and these proteomic characteristics were very much similar to those in ES cells indicating that multipotency of mGS and ES cells is based on a common molecular event(s). Protein identification by mass spectrometry revealed that these proteins were functionally involved in cell signaling, transcription factors, metabolism, and protein folding. The identified proteins in the present study may thus reveal its biological characteristics and functional property in self-renewal and multipotency.
AB - Germline stem (GS) cells can only differentiate into germline cells, while multipotent germ stem (mGS) cells, like embryonic stem (ES) cells, can differentiate into various somatic cells and tissues. The proteomic profiles in GS and mGS cells were compared by two-dimensional gel electrophoresis. Ten down-regulated and 16 up-regulated proteins were differentially expressed in mGS cells in comparison to GS cells, and these proteomic characteristics were very much similar to those in ES cells indicating that multipotency of mGS and ES cells is based on a common molecular event(s). Protein identification by mass spectrometry revealed that these proteins were functionally involved in cell signaling, transcription factors, metabolism, and protein folding. The identified proteins in the present study may thus reveal its biological characteristics and functional property in self-renewal and multipotency.
KW - Embryonal carcinoma cells
KW - Embryonic germ cells
KW - Embryonic stem cells
KW - Germline stem cells
KW - Multipotency
KW - Proteomics
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U2 - 10.1016/j.bbrc.2006.12.025
DO - 10.1016/j.bbrc.2006.12.025
M3 - Article
C2 - 17188235
AN - SCOPUS:33845886114
SN - 0006-291X
VL - 353
SP - 259
EP - 267
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -