TY - JOUR
T1 - A composite of hydroxyapatite with electrospun biodegradable nanofibers as a tissue engineering material
AU - Ito, Yoshihiro
AU - Hasuda, Hirokazu
AU - Kamitakahara, Masanobu
AU - Ohtsuki, Chikara
AU - Tanihara, Masao
AU - Kang, Inn Kyu
AU - Kwon, Oh Hyeong
N1 - Funding Information:
Y. I. would like to thank Prof. A. Kishida at Tokyo Medical and Dental University for valuable comments on this study. This study was supported in part by grants from the Japanese Ministry of Education, Culture, Sports, Science and Technology (no. 14380406), the Uehara Memorial Foundation, and the Korean Research Foundation (KRF-2002-042-D00045).
PY - 2005
Y1 - 2005
N2 - Biodegradable and biocompatible poly(3-hydroxybutyrate-co-3- hydroxyvalerate) (PHBV), a copolymer of microbial polyester, was fabricated as a nanofibrous film by electrospinning and composited with hydroxyapatite (HAp) by soaking in simulated body fluid. Compared with a PHBV cast (flat) film, the electrospun PHBV nanofibrous film was hydrophobic. However, after HAp deposition, both of the surfaces were extremely hydrophilic. The degradation rate of HAp/PHBV nanofibrous films in the presence of polyhydroxybutyrate depolymerase was very fast. Nanofiber formation increased the specific surface area and HAp enhanced the invasion of enzyme into the film by increasing surface hydrophilicity. The surface of the nanofibrous film showed enhanced cell adhesion over that of the flat film, although cell adhesion was not significantly affected by the combination with HAp.
AB - Biodegradable and biocompatible poly(3-hydroxybutyrate-co-3- hydroxyvalerate) (PHBV), a copolymer of microbial polyester, was fabricated as a nanofibrous film by electrospinning and composited with hydroxyapatite (HAp) by soaking in simulated body fluid. Compared with a PHBV cast (flat) film, the electrospun PHBV nanofibrous film was hydrophobic. However, after HAp deposition, both of the surfaces were extremely hydrophilic. The degradation rate of HAp/PHBV nanofibrous films in the presence of polyhydroxybutyrate depolymerase was very fast. Nanofiber formation increased the specific surface area and HAp enhanced the invasion of enzyme into the film by increasing surface hydrophilicity. The surface of the nanofibrous film showed enhanced cell adhesion over that of the flat film, although cell adhesion was not significantly affected by the combination with HAp.
KW - Biodegradable polymer
KW - Cell attachment
KW - Electrospun nanofibers
KW - Hydroxyapatite
KW - Tissue engineering
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U2 - 10.1263/jbb.100.43
DO - 10.1263/jbb.100.43
M3 - Article
C2 - 16233849
AN - SCOPUS:27644495665
SN - 1389-1723
VL - 100
SP - 43
EP - 49
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
IS - 1
ER -