TY - JOUR
T1 - A convenient method for preparation of high-purity, Amadori-glycated phosphatidylethanolamine and its prooxidant effect
AU - Miyazawa, Teruo
AU - Oak, Jeong Ho
AU - Nakagawa, Kiyotaka
PY - 2005
Y1 - 2005
N2 - Phospholipid-linked glycation has been implicated to play an important role in lipid peroxidation associated with several conditions, such as normal aging and diabetes. In this study, we established a convenient method for the preparation of a high-purity, Amadori-glycated phosphatidylethanolamine (Amadori-PE) standard in a methanol system. We evaluated the pro-oxidant effect of the Amadori-PE standard. When Amadori-PE was incubated in a micellar and liposome system in the presence of metal ion, a remarkable increase of lipid peroxidation was observed in both systems. In addition, the Amadori-PE-induced lipid peroxidation was effectively inhibited by superoxide dismutase, mannitol, catalase, and EDTA. These results indicate that Amadori-PE is a considerable risk factor that may cause membrane lipid peroxidation in the pathogenesis of diabetes and aging.
AB - Phospholipid-linked glycation has been implicated to play an important role in lipid peroxidation associated with several conditions, such as normal aging and diabetes. In this study, we established a convenient method for the preparation of a high-purity, Amadori-glycated phosphatidylethanolamine (Amadori-PE) standard in a methanol system. We evaluated the pro-oxidant effect of the Amadori-PE standard. When Amadori-PE was incubated in a micellar and liposome system in the presence of metal ion, a remarkable increase of lipid peroxidation was observed in both systems. In addition, the Amadori-PE-induced lipid peroxidation was effectively inhibited by superoxide dismutase, mannitol, catalase, and EDTA. These results indicate that Amadori-PE is a considerable risk factor that may cause membrane lipid peroxidation in the pathogenesis of diabetes and aging.
KW - Amadori product
KW - Diabetes mellitus
KW - Glycation
KW - Lipid peroxidation
KW - Phosphatidylethanolamine
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U2 - 10.1196/annals.1333.033
DO - 10.1196/annals.1333.033
M3 - Article
C2 - 16037248
AN - SCOPUS:23744448894
SN - 0077-8923
VL - 1043
SP - 276
EP - 279
JO - Annals of the New York Academy of Sciences
JF - Annals of the New York Academy of Sciences
ER -
