A dimeric pyrrocidine from Neonectria ramulariae is an inhibitor of prolyl oligopeptidase

Yoshihito Shiono, Azusa Kosukegawa, Takuya Koseki, Tetsuya Murayama, Eunsang Kwon, Shota Uesugi, Ken Ichi Kimura

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Four novel alkaloids, bispyrrocidine (5), the epoxy derivative of pyrrocidine B (6), 19-O-methyl-pyrrrocidine B (7) and 19-O-ethyl-pyrrrocidine B (8) were isolated from the endophytic fungus Neonectria ramulariae Wollenw KS-246. Their structures were elucidated using 1D- and 2D-NMR spectroscopy. Compound 6 exhibited cytotoxicity against HL60 cells (IC 50 4.6 μM), whereas 5 showed specific inhibitory activity against prolyl oligopeptidase (IC 50 2.6 μM) in a non-competitive manner.

Original languageEnglish
Pages (from-to)91-95
Number of pages5
JournalPhytochemistry Letters
Volume5
Issue number1
DOIs
Publication statusPublished - 2012 Mar

Keywords

  • Bispyrrocidine
  • Endophyte
  • Neonectria ramulariae
  • Prolyl oligopeptidase inhibitor

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Agronomy and Crop Science
  • Plant Science

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