A double chaperone function of the sHsp genes against heat-based environmental adversity in the soil-dwelling leaf beetles

Elizabeth Atungulu; Hiromasa Tanaka; Kosuke Fujita; Kei Ichiro Yamamoto; Mutsumi Sakata; Erika Sato; Michihiro Hara; Tetsuro Yamashita; Koichi Suzuki

A double chaperone function of the sHsp genes against heat-based environmental adversity in the soil-dwelling leaf beetles

Elizabeth Atungulu, Hiromasa Tanaka, Kosuke Fujita, Kei Ichiro Yamamoto, Mutsumi Sakata, Erika Sato, Michihiro Hara, Tetsuro Yamashita, Koichi Suzuki

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

Small heat shock proteins (sHsps), which are constitutively expressed in the absence of stress during development in many organisms, have been correlated with not only enhanced thermoresistance but also the developmental, tissue-, and cell-specific induction and expression. This study demonstrates the constitutive sHsps 21 and 23 of an entomoresouce, the leaf beetle, Gastrophysa atrocyanea, display a double chaperone function in vitro. The RNAi suppression of two sHsp genes in adults resulted in decreased thermoresistance. A strong correlation was observed between the in vitro chaperone function and in vivo thermotolerance analysis, and the results support a possible double chaperone function critical for the survival of the leaf beetles against higher temperatures.

Original language	English
Pages (from-to)	15-22
Number of pages	8
Journal	Journal of Insect Biotechnology and Sericology
Volume	75
Issue number	1
Publication status	Published - 2006 Feb
Externally published	Yes

Keywords

Adult diapause
Double chaperone function
Gastrophysa atrocyanea
RNAi
sHsps

ASJC Scopus subject areas

Biotechnology
Applied Microbiology and Biotechnology
Business, Management and Accounting(all)
Agricultural and Biological Sciences(all)
Insect Science
Industrial and Manufacturing Engineering

Cite this

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title = "A double chaperone function of the sHsp genes against heat-based environmental adversity in the soil-dwelling leaf beetles",

abstract = "Small heat shock proteins (sHsps), which are constitutively expressed in the absence of stress during development in many organisms, have been correlated with not only enhanced thermoresistance but also the developmental, tissue-, and cell-specific induction and expression. This study demonstrates the constitutive sHsps 21 and 23 of an entomoresouce, the leaf beetle, Gastrophysa atrocyanea, display a double chaperone function in vitro. The RNAi suppression of two sHsp genes in adults resulted in decreased thermoresistance. A strong correlation was observed between the in vitro chaperone function and in vivo thermotolerance analysis, and the results support a possible double chaperone function critical for the survival of the leaf beetles against higher temperatures.",

keywords = "Adult diapause, Double chaperone function, Gastrophysa atrocyanea, RNAi, sHsps",

author = "Elizabeth Atungulu and Hiromasa Tanaka and Kosuke Fujita and Yamamoto, {Kei Ichiro} and Mutsumi Sakata and Erika Sato and Michihiro Hara and Tetsuro Yamashita and Koichi Suzuki",

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AU - Atungulu, Elizabeth

AU - Tanaka, Hiromasa

AU - Fujita, Kosuke

AU - Yamamoto, Kei Ichiro

AU - Sakata, Mutsumi

AU - Sato, Erika

AU - Hara, Michihiro

AU - Yamashita, Tetsuro

AU - Suzuki, Koichi

PY - 2006/2

Y1 - 2006/2

N2 - Small heat shock proteins (sHsps), which are constitutively expressed in the absence of stress during development in many organisms, have been correlated with not only enhanced thermoresistance but also the developmental, tissue-, and cell-specific induction and expression. This study demonstrates the constitutive sHsps 21 and 23 of an entomoresouce, the leaf beetle, Gastrophysa atrocyanea, display a double chaperone function in vitro. The RNAi suppression of two sHsp genes in adults resulted in decreased thermoresistance. A strong correlation was observed between the in vitro chaperone function and in vivo thermotolerance analysis, and the results support a possible double chaperone function critical for the survival of the leaf beetles against higher temperatures.

AB - Small heat shock proteins (sHsps), which are constitutively expressed in the absence of stress during development in many organisms, have been correlated with not only enhanced thermoresistance but also the developmental, tissue-, and cell-specific induction and expression. This study demonstrates the constitutive sHsps 21 and 23 of an entomoresouce, the leaf beetle, Gastrophysa atrocyanea, display a double chaperone function in vitro. The RNAi suppression of two sHsp genes in adults resulted in decreased thermoresistance. A strong correlation was observed between the in vitro chaperone function and in vivo thermotolerance analysis, and the results support a possible double chaperone function critical for the survival of the leaf beetles against higher temperatures.

KW - Adult diapause

KW - Double chaperone function

KW - Gastrophysa atrocyanea

KW - RNAi

KW - sHsps

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A double chaperone function of the sHsp genes against heat-based environmental adversity in the soil-dwelling leaf beetles

Abstract

Keywords

ASJC Scopus subject areas

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