A freestanding microfluidic-based thermocouple biosensor for enzyme-catalyzed reaction analysis

Zhuqing Wang, Mitsuteru Kimura, Naoki Inomata, Takahito Ono

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

2 Citations (Scopus)

Abstract

This research reports a multifunctional thermal biosensor that has thermocouple structures integrated with freestanding microfluidic channels for bio-sensing based on enzyme-catalyzed reactions. The free-standing SU-8 polymer-based microfluidic measurement chamber allows sensitive measurement of small volumes of liquid samples and reduces heat loss to improve the sensitivity. The fabricated thermocouple biosensor is capable of enzyme-catalyzed reaction detection for healthcare applications. Reactive enzymes are immobilized on microspheres packed in the chambers. The fabricated thermocouple biosensor shows a sensitivity of approximately 0.5 V/W and a thermal time constant of less than 100ms. The Seebeck-coefficient of thermocouple biosensor obtains a sensitivity of 1.1mV/°C with the measurement of IR thermometry. The results demonstrate that the sensor was capable of enzyme-catalyzed reaction detection for healthcare application.

Original languageEnglish
Title of host publication2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages58-61
Number of pages4
ISBN (Electronic)9781509019472
DOIs
Publication statusPublished - 2016 Nov 28
Event11th IEEE Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016 - Sendai, Japan
Duration: 2016 Apr 172016 Apr 20

Publication series

Name2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016

Conference

Conference11th IEEE Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016
Country/TerritoryJapan
CitySendai
Period16/4/1716/4/20

Keywords

  • Biosensor
  • Enzyme immobilization
  • Fluidic struture
  • Thermocouple

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