TY - JOUR
T1 - A gene encoding pentatricopeptide repeat protein partially restores fertility in RT98-Type cytoplasmic male-sterile rice
AU - Igarashi, Keisuke
AU - Kazama, Tomohiko
AU - Toriyama, Kinya
N1 - Funding Information:
This work was supported by the Ministry of Education, Culture, Sports, Science and Technology (Japan)/Japan Society for the Promotion of Science [KAKENHI grant Nos. 2338002, 24117502 and 26292002]; the Science and technology research promotion program for agriculture, forestry, fisheries and food industry [grant No. 26010A]; the Ministry of Agriculture, Forestry and Fisheries of Japan [a program for the Promotion of Basic and Applied Research for Innovations in Bio-oriented Industry, Genomics for Agricultural Innovation QTL-4008]; Japan Society for the promotion of Science for Young Scientists [Research Fellowship to K.I.].
Publisher Copyright:
© The Author 2016. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved.
PY - 2016/10
Y1 - 2016/10
N2 - Cytoplasmic male sterility (CMS) lines in rice, which have the cytoplasm of a wild species and the nuclear genome of cultivated rice, are of value for the study of genetic interactions between the mitochondrial and nuclear genomes. The RT98-Type CMS line RT98A and the fertility restorer line RT98C carry the cytoplasm of the wild species Oryza rufipogon and the nuclear genome of the Taichung 65 cultivar (Oryza sativa L.). Based on a classical crossing experiment, fertility is reported to be restored gametophytically by the presence of a tentative single gene, designated Rf98, which is derived from the cytoplasm donor. Fine mapping of Rf98 revealed that at least two genes, which are closely positioned, are required for complete fertility restoration in RT98A. Here, we identified seven pentatricopeptide repeat (PPR) genes that are located within a 170 kb region as candidates for Rf98. Complementation tests revealed that the introduction of one of these PPR genes, PPR762, resulted in the partial recovery of fertility with a seed setting rate up to 9.3%. We conclude that PPR762 is an essential fertility restorer gene for RT98-Type CMS. The low rate of seed setting suggested that some other genes near the Rf98 locus are also necessary for the full recovery of seed setting.
AB - Cytoplasmic male sterility (CMS) lines in rice, which have the cytoplasm of a wild species and the nuclear genome of cultivated rice, are of value for the study of genetic interactions between the mitochondrial and nuclear genomes. The RT98-Type CMS line RT98A and the fertility restorer line RT98C carry the cytoplasm of the wild species Oryza rufipogon and the nuclear genome of the Taichung 65 cultivar (Oryza sativa L.). Based on a classical crossing experiment, fertility is reported to be restored gametophytically by the presence of a tentative single gene, designated Rf98, which is derived from the cytoplasm donor. Fine mapping of Rf98 revealed that at least two genes, which are closely positioned, are required for complete fertility restoration in RT98A. Here, we identified seven pentatricopeptide repeat (PPR) genes that are located within a 170 kb region as candidates for Rf98. Complementation tests revealed that the introduction of one of these PPR genes, PPR762, resulted in the partial recovery of fertility with a seed setting rate up to 9.3%. We conclude that PPR762 is an essential fertility restorer gene for RT98-Type CMS. The low rate of seed setting suggested that some other genes near the Rf98 locus are also necessary for the full recovery of seed setting.
KW - Cytoplasmic male sterility (CMS)
KW - Oryza rufipogon
KW - Pentatricopeptide repeat protein
KW - Restorer of fertility (Rf)
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U2 - 10.1093/pcp/pcw135
DO - 10.1093/pcp/pcw135
M3 - Article
C2 - 27498808
AN - SCOPUS:84995551048
SN - 0032-0781
VL - 57
SP - 2187
EP - 2193
JO - Plant and Cell Physiology
JF - Plant and Cell Physiology
IS - 10
ER -