TY - JOUR
T1 - A gonad-expressed opsin mediates light-induced spawning in the jellyfish clytia
AU - Quiroga Artigas, Gonzalo
AU - Lapébie, Pascal
AU - Leclère, Lucas
AU - Takeda, Noriyo
AU - Deguchi, Ryusaku
AU - Jékely, Gáspár
AU - Momose, Tsuyoshi
AU - Houliston, Evelyn
N1 - Funding Information:
European Commission FP7-PEOPLE-2012-ITN 317172 (NEPTUNE) Gáspár Jékely Evelyn Houliston Centre National de la Recherche Scientifique Evelyn Houliston Agence Nationale de la Re-cherche ANR-13-BSV2-0008-01 ("OOCAMP") Evelyn Houliston.
Funding Information:
We gratefully acknowledge the technical assistance of J Uveira and S Chevalier, as well as L Gissat and Sophie Collet for animal maintenance. We thank M Khamla for the graphics in Figure 1A, J-P Concordet (MNHN Paris) for generously providing Cas9 protein, M Gühmann for monochromator training, C Rouvière for image quantification methods and all our laboratory and NEPTUNE colleagues for stimulating and critical discussions. Funding was provided by the Marie Curie ITN NEPTUNE, French ANR grant OOCAMP, EMBRC-Fr infrastructure development funding and core CNRS funding to the LBDV. Microscopy was performed at the PIV imaging platform.
Publisher Copyright:
© Quiroga Artigas et al.
PY - 2018/1/5
Y1 - 2018/1/5
N2 - Across the animal kingdom, environmental light cues are widely involved in regulating gamete release, but the molecular and cellular bases of the photoresponsive mechanisms are poorly understood. In hydrozoan jellyfish, spawning is triggered by dark-light or light-dark transitions acting on the gonad, and is mediated by oocyte maturation-inducing neuropeptide hormones (MIHs) released from the ectoderm. We determined in Clytia hemisphaerica that blue-cyan light triggers spawning in isolated gonads. A candidate opsin (Opsin9) was found co-expressed with MIH within specialised ectodermal cells. Opsin9 knockout jellyfish generated by CRISPR/Cas9 failed to undergo oocyte maturation and spawning, a phenotype reversible by synthetic MIH. Gamete maturation and release in Clytia is thus regulated by gonadal photosensory-neurosecretory cells that secrete MIH in response to light via Opsin9. Similar cells in ancestral eumetazoans may have allowed tissue-level photo-regulation of diverse behaviours, a feature elaborated in cnidarians in parallel with expansion of the opsin gene family.
AB - Across the animal kingdom, environmental light cues are widely involved in regulating gamete release, but the molecular and cellular bases of the photoresponsive mechanisms are poorly understood. In hydrozoan jellyfish, spawning is triggered by dark-light or light-dark transitions acting on the gonad, and is mediated by oocyte maturation-inducing neuropeptide hormones (MIHs) released from the ectoderm. We determined in Clytia hemisphaerica that blue-cyan light triggers spawning in isolated gonads. A candidate opsin (Opsin9) was found co-expressed with MIH within specialised ectodermal cells. Opsin9 knockout jellyfish generated by CRISPR/Cas9 failed to undergo oocyte maturation and spawning, a phenotype reversible by synthetic MIH. Gamete maturation and release in Clytia is thus regulated by gonadal photosensory-neurosecretory cells that secrete MIH in response to light via Opsin9. Similar cells in ancestral eumetazoans may have allowed tissue-level photo-regulation of diverse behaviours, a feature elaborated in cnidarians in parallel with expansion of the opsin gene family.
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U2 - 10.7554/eLife.29555
DO - 10.7554/eLife.29555
M3 - Article
AN - SCOPUS:85041370959
SN - 2050-084X
VL - 7
JO - eLife
JF - eLife
M1 - e29555
ER -