@article{918c06d8f23147e3b784c5300600e5a5,
title = "A High-Resolution Map of SBP1 Interactomes in Plasmodium falciparum-infected Erythrocytes",
abstract = "The pathogenesis of malaria parasites depends on host erythrocyte modifications that are facilitated by parasite proteins exported to the host cytoplasm. These exported proteins form a trafficking complex in the host cytoplasm that transports virulence determinants to the erythrocyte surface; this complex is thus essential for malaria virulence. Here, we report a comprehensive interaction network map of this complex. We developed authentic, unbiased, highly sensitive proteomic approaches to determine the proteins that interact with a core component of the complex, SBP1 (skeleton-binding protein 1). SBP1 interactomes revealed numerous exported proteins and potential interactors associated with SBP1 intracellular trafficking. We identified several host-parasite protein interactions and linked the exported protein MAL8P1.4 to Plasmodium falciparum virulence in infected erythrocytes. Our study highlights the complicated interplay between parasite and host proteins in the host cytoplasm and provides an interaction dataset connecting dozens of exported proteins required for P. falciparum virulence.",
keywords = "Microbiology, Parasitology, Proteomics",
author = "Ryo Takano and Hiroko Kozuka-Hata and Daisuke Kondoh and Hiroki Bochimoto and Masaaki Oyama and Kentaro Kato",
note = "Funding Information: R.T. is supported by Research Fellowships from the Japan Society for the Promotion of Science for Young Scientists from the MEXT of Japan. Funding Information: We thank Susan Watson for editing the manuscript. We thank Dr. Takafumi Tsuboi (Ehime University, Japan) for providing rabbit anti-EXP2 and mouse and rabbit anti-SBP1 antibodies, and Dr. Alan F. Cowman (Walter Eliza Hall Institute for Medical Research, Australia) for the pARL1 transfection plasmid. We also thank Drs. Hitoshi Takemae, Tatsuki Sugi, and Takeshi Q. Tanaka (Obihiro University of Agriculture and Veterinary Medicine, Japan) and Dr. Motomi Torii (Ehime University, Japan) for technical advice and useful discussions. This work was supported by Grants-in-Aid for Young Scientists and Scientific Research on Innovative Areas (3308) from the Ministry of Education, Culture, Science, Sports, and Technology (MEXT) of Japan, the Program to Disseminate Tenure Tracking System from the Japan Science and Technology Agency (JST), the Takeda Science Foundation , and the Suhara Memorial Foundation . Funding Information: We thank Susan Watson for editing the manuscript. We thank Dr. Takafumi Tsuboi (Ehime University, Japan) for providing rabbit anti-EXP2 and mouse and rabbit anti-SBP1 antibodies, and Dr. Alan F. Cowman (Walter Eliza Hall Institute for Medical Research, Australia) for the pARL1 transfection plasmid. We also thank Drs. Hitoshi Takemae, Tatsuki Sugi, and Takeshi Q. Tanaka (Obihiro University of Agriculture and Veterinary Medicine, Japan) and Dr. Motomi Torii (Ehime University, Japan) for technical advice and useful discussions. This work was supported by Grants-in-Aid for Young Scientists and Scientific Research on Innovative Areas (3308) from the Ministry of Education, Culture, Science, Sports, and Technology (MEXT) of Japan, the Program to Disseminate Tenure Tracking System from the Japan Science and Technology Agency (JST), the Takeda Science Foundation, and the Suhara Memorial Foundation. R.T. is supported by Research Fellowships from the Japan Society for the Promotion of Science for Young Scientists from the MEXT of Japan. R.T. conceived and designed the project, performed the experiments, analyzed the data, and prepared and wrote the manuscript. H.K.-H. and M.O. performed the mass spectrometry analyses. D.K. and H.B. performed the electron microscopic analyses. K.K. supervised and oversaw the project. All authors approved the final manuscript. The authors declare that no competing interests exist. Publisher Copyright: {\textcopyright} 2019 The Author(s)",
year = "2019",
month = sep,
day = "27",
doi = "10.1016/j.isci.2019.07.035",
language = "English",
volume = "19",
pages = "703--714",
journal = "iScience",
issn = "2589-0042",
publisher = "Elsevier Inc.",
}
