TY - GEN
T1 - A light-addressable potentiometric sensor system for fast, simultaneous and spatial detection of the metabolic activity of biological cells
AU - Wagner, T.
AU - Molina, R.
AU - Biselli, M.
AU - Canzoneri, M.
AU - Schnitzler, T.
AU - Yoshinobu, T.
AU - Scheming, M. J.
PY - 2007
Y1 - 2007
N2 - The metabolic activity of cells can be utilised to indicate the general reaction of them towards the cell environment. Hence, the influence of a specific reagent (e.g., drugs, toxins, growth factors, etc.) in the cell-culture medium can be determined by recording the metabolic activity. The light-addressable potentiometric sensor (LAPS), a field-effect-based chemical sensor, provides a promising technology platform to record the metabolic activity of cells with a spatial resolution. In this work, a portable LAPS device will be introduced which is capable of measuring at 16 different locations within the sensor surface simultaneously. The cultivation and recording of the metabolic activity of CHO (Chinese hamster ovary) cells within the LAPS set-up will be demonstrated.
AB - The metabolic activity of cells can be utilised to indicate the general reaction of them towards the cell environment. Hence, the influence of a specific reagent (e.g., drugs, toxins, growth factors, etc.) in the cell-culture medium can be determined by recording the metabolic activity. The light-addressable potentiometric sensor (LAPS), a field-effect-based chemical sensor, provides a promising technology platform to record the metabolic activity of cells with a spatial resolution. In this work, a portable LAPS device will be introduced which is capable of measuring at 16 different locations within the sensor surface simultaneously. The cultivation and recording of the metabolic activity of CHO (Chinese hamster ovary) cells within the LAPS set-up will be demonstrated.
KW - Cells
KW - Field effect
KW - Light-addressable potentiometric sensor
KW - Metabolism
UR - http://www.scopus.com/inward/record.url?scp=50049128689&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=50049128689&partnerID=8YFLogxK
U2 - 10.1109/SENSOR.2007.4300328
DO - 10.1109/SENSOR.2007.4300328
M3 - Conference contribution
AN - SCOPUS:50049128689
SN - 1424408423
SN - 9781424408429
T3 - TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems
SP - 1107
EP - 1110
BT - TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems
T2 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS '07
Y2 - 10 June 2007 through 14 June 2007
ER -