A linkage map for sugi (Cryptomeria japonica) based on RFLP, RAPD, and isozyme loci

Y. Mukai; Y. Suyama; Y. Tsumura; T. Kawahara; H. Yoshimaru; T. Kondo; N. Tomaru; N. Kuramoto; M. Murai

doi:10.1007/BF00222019

A linkage map for sugi (Cryptomeria japonica) based on RFLP, RAPD, and isozyme loci

Y. Mukai, Y. Suyama, Y. Tsumura, T. Kawahara, H. Yoshimaru, T. Kondo, N. Tomaru, N. Kuramoto, M. Murai

AGR - Agricultural Bioscience

Research output: Contribution to journal › Article › peer-review

50 Citations (Scopus)

Abstract

A linkage map for sugi was constructed on the basis of restriction fragment length polymorphism (RFLP), random amplified polymorphic DNA (RAPD), and isozyme loci using a three-generation pedigree prepared for genetic analysis of heartwood color. A total of 128 RFLP (123 cDNA and 5 genomic probes), 33 RAPD, 2 isozyme, and 1 morphological (dwarf) loci segregated in 73 progeny. Of the 164 segregating loci, 145 loci were distributed in 20 linkage groups. Of these loci, 91 with confirmed map positions were assigned to 13 linkage groups, covering a total of 887.3 cM. A clustering of markers with distorted segregation was observed in 6 linkage groups. In the four clusters, distortions with a reduction in the number of homozygotes from one parent only were found.

Original language	English
Pages (from-to)	835-840
Number of pages	6
Journal	Theoretical And Applied Genetics
Volume	90
Issue number	6
DOIs	https://doi.org/10.1007/BF00222019
Publication status	Published - 1995 May

Keywords

Linkage map
RAPD
RFLP
Sugi (Cryptomeria japonica)
Three-generation pedigree

Access to Document

10.1007/BF00222019

Cite this

@article{28333278ffc24365b445ffd1aafc775a,

title = "A linkage map for sugi (Cryptomeria japonica) based on RFLP, RAPD, and isozyme loci",

abstract = "A linkage map for sugi was constructed on the basis of restriction fragment length polymorphism (RFLP), random amplified polymorphic DNA (RAPD), and isozyme loci using a three-generation pedigree prepared for genetic analysis of heartwood color. A total of 128 RFLP (123 cDNA and 5 genomic probes), 33 RAPD, 2 isozyme, and 1 morphological (dwarf) loci segregated in 73 progeny. Of the 164 segregating loci, 145 loci were distributed in 20 linkage groups. Of these loci, 91 with confirmed map positions were assigned to 13 linkage groups, covering a total of 887.3 cM. A clustering of markers with distorted segregation was observed in 6 linkage groups. In the four clusters, distortions with a reduction in the number of homozygotes from one parent only were found.",

keywords = "Linkage map, RAPD, RFLP, Sugi (Cryptomeria japonica), Three-generation pedigree",

author = "Y. Mukai and Y. Suyama and Y. Tsumura and T. Kawahara and H. Yoshimaru and T. Kondo and N. Tomaru and N. Kuramoto and M. Murai",

year = "1995",

month = may,

doi = "10.1007/BF00222019",

language = "English",

volume = "90",

pages = "835--840",

journal = "Theoretical And Applied Genetics",

issn = "0040-5752",

publisher = "Springer Verlag",

number = "6",

}

TY - JOUR

T1 - A linkage map for sugi (Cryptomeria japonica) based on RFLP, RAPD, and isozyme loci

AU - Mukai, Y.

AU - Suyama, Y.

AU - Tsumura, Y.

AU - Kawahara, T.

AU - Yoshimaru, H.

AU - Kondo, T.

AU - Tomaru, N.

AU - Kuramoto, N.

AU - Murai, M.

PY - 1995/5

Y1 - 1995/5

N2 - A linkage map for sugi was constructed on the basis of restriction fragment length polymorphism (RFLP), random amplified polymorphic DNA (RAPD), and isozyme loci using a three-generation pedigree prepared for genetic analysis of heartwood color. A total of 128 RFLP (123 cDNA and 5 genomic probes), 33 RAPD, 2 isozyme, and 1 morphological (dwarf) loci segregated in 73 progeny. Of the 164 segregating loci, 145 loci were distributed in 20 linkage groups. Of these loci, 91 with confirmed map positions were assigned to 13 linkage groups, covering a total of 887.3 cM. A clustering of markers with distorted segregation was observed in 6 linkage groups. In the four clusters, distortions with a reduction in the number of homozygotes from one parent only were found.

AB - A linkage map for sugi was constructed on the basis of restriction fragment length polymorphism (RFLP), random amplified polymorphic DNA (RAPD), and isozyme loci using a three-generation pedigree prepared for genetic analysis of heartwood color. A total of 128 RFLP (123 cDNA and 5 genomic probes), 33 RAPD, 2 isozyme, and 1 morphological (dwarf) loci segregated in 73 progeny. Of the 164 segregating loci, 145 loci were distributed in 20 linkage groups. Of these loci, 91 with confirmed map positions were assigned to 13 linkage groups, covering a total of 887.3 cM. A clustering of markers with distorted segregation was observed in 6 linkage groups. In the four clusters, distortions with a reduction in the number of homozygotes from one parent only were found.

KW - Linkage map

KW - RAPD

KW - RFLP

KW - Sugi (Cryptomeria japonica)

KW - Three-generation pedigree

UR - http://www.scopus.com/inward/record.url?scp=0000911323&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0000911323&partnerID=8YFLogxK

U2 - 10.1007/BF00222019

DO - 10.1007/BF00222019

M3 - Article

AN - SCOPUS:0000911323

SN - 0040-5752

VL - 90

SP - 835

EP - 840

JO - Theoretical And Applied Genetics

JF - Theoretical And Applied Genetics

IS - 6

ER -

A linkage map for sugi (Cryptomeria japonica) based on RFLP, RAPD, and isozyme loci

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this