A microbial chip combined with scanning electrochemical microscopy

Takatoshi Kaya; Matsuhiko Nishizawa; Tomayuki Yasukawa; Masashi Nishiguchi; Tooru Onouchi; Tomokazu Matsue

doi:10.1002/bit.10085

A microbial chip combined with scanning electrochemical microscopy

Takatoshi Kaya, Matsuhiko Nishizawa, Tomayuki Yasukawa, Masashi Nishiguchi, Tooru Onouchi, Tomokazu Matsue

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

A microbial chip for bioassay was fabricated and its performance was characterized by scanning electrochemical microscopy (SECM). The microbial chip was prepared by spotting a suspension of Escherichia coil on a polystyrene substrate by using a glass capillary pen. The respiration activity of the E. coli spot was imaged with SECM by mapping the oxygen concentration around the spot. The SECM images of the microbial chips clearly showed spots with lower reduction currents, indicating that E. coli in the spots uptake oxygen by respiration. The bactericidal effects of antibiotics (streptomycin and ampicillin) were measured using the E. coli-based microbial chip, and discussed in comparison with the minimum inhibitory concentration (MIC) determined by an agar plate dilution method.

Original language	English
Pages (from-to)	391-394
Number of pages	4
Journal	Biotechnology and Bioengineering
Volume	76
Issue number	4
DOIs	https://doi.org/10.1002/bit.10085
Publication status	Published - 2001 Nov 8

Keywords

Bactericide
Microbial chip
Respiration activity
Scanning electrochemical microscopy (SECM)

Access to Document

10.1002/bit.10085

Cite this

@article{6ff5a50340f94e02a0c4a0da5dac72f7,

title = "A microbial chip combined with scanning electrochemical microscopy",

abstract = "A microbial chip for bioassay was fabricated and its performance was characterized by scanning electrochemical microscopy (SECM). The microbial chip was prepared by spotting a suspension of Escherichia coil on a polystyrene substrate by using a glass capillary pen. The respiration activity of the E. coli spot was imaged with SECM by mapping the oxygen concentration around the spot. The SECM images of the microbial chips clearly showed spots with lower reduction currents, indicating that E. coli in the spots uptake oxygen by respiration. The bactericidal effects of antibiotics (streptomycin and ampicillin) were measured using the E. coli-based microbial chip, and discussed in comparison with the minimum inhibitory concentration (MIC) determined by an agar plate dilution method.",

keywords = "Bactericide, Microbial chip, Respiration activity, Scanning electrochemical microscopy (SECM)",

author = "Takatoshi Kaya and Matsuhiko Nishizawa and Tomayuki Yasukawa and Masashi Nishiguchi and Tooru Onouchi and Tomokazu Matsue",

year = "2001",

month = nov,

day = "8",

doi = "10.1002/bit.10085",

language = "English",

volume = "76",

pages = "391--394",

journal = "Biotechnology and Bioengineering",

issn = "0006-3592",

publisher = "Wiley-VCH Verlag",

number = "4",

}

TY - JOUR

T1 - A microbial chip combined with scanning electrochemical microscopy

AU - Kaya, Takatoshi

AU - Nishizawa, Matsuhiko

AU - Yasukawa, Tomayuki

AU - Nishiguchi, Masashi

AU - Onouchi, Tooru

AU - Matsue, Tomokazu

PY - 2001/11/8

Y1 - 2001/11/8

N2 - A microbial chip for bioassay was fabricated and its performance was characterized by scanning electrochemical microscopy (SECM). The microbial chip was prepared by spotting a suspension of Escherichia coil on a polystyrene substrate by using a glass capillary pen. The respiration activity of the E. coli spot was imaged with SECM by mapping the oxygen concentration around the spot. The SECM images of the microbial chips clearly showed spots with lower reduction currents, indicating that E. coli in the spots uptake oxygen by respiration. The bactericidal effects of antibiotics (streptomycin and ampicillin) were measured using the E. coli-based microbial chip, and discussed in comparison with the minimum inhibitory concentration (MIC) determined by an agar plate dilution method.

AB - A microbial chip for bioassay was fabricated and its performance was characterized by scanning electrochemical microscopy (SECM). The microbial chip was prepared by spotting a suspension of Escherichia coil on a polystyrene substrate by using a glass capillary pen. The respiration activity of the E. coli spot was imaged with SECM by mapping the oxygen concentration around the spot. The SECM images of the microbial chips clearly showed spots with lower reduction currents, indicating that E. coli in the spots uptake oxygen by respiration. The bactericidal effects of antibiotics (streptomycin and ampicillin) were measured using the E. coli-based microbial chip, and discussed in comparison with the minimum inhibitory concentration (MIC) determined by an agar plate dilution method.

KW - Bactericide

KW - Microbial chip

KW - Respiration activity

KW - Scanning electrochemical microscopy (SECM)

UR - http://www.scopus.com/inward/record.url?scp=0035829829&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035829829&partnerID=8YFLogxK

U2 - 10.1002/bit.10085

DO - 10.1002/bit.10085

M3 - Article

C2 - 11745166

AN - SCOPUS:0035829829

SN - 0006-3592

VL - 76

SP - 391

EP - 394

JO - Biotechnology and Bioengineering

JF - Biotechnology and Bioengineering

IS - 4

ER -

A microbial chip combined with scanning electrochemical microscopy

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this