A microbial chip combined with scanning electrochemical microscopy

Takatoshi Kaya, Matsuhiko Nishizawa, Tomayuki Yasukawa, Masashi Nishiguchi, Tooru Onouchi, Tomokazu Matsue

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)


A microbial chip for bioassay was fabricated and its performance was characterized by scanning electrochemical microscopy (SECM). The microbial chip was prepared by spotting a suspension of Escherichia coil on a polystyrene substrate by using a glass capillary pen. The respiration activity of the E. coli spot was imaged with SECM by mapping the oxygen concentration around the spot. The SECM images of the microbial chips clearly showed spots with lower reduction currents, indicating that E. coli in the spots uptake oxygen by respiration. The bactericidal effects of antibiotics (streptomycin and ampicillin) were measured using the E. coli-based microbial chip, and discussed in comparison with the minimum inhibitory concentration (MIC) determined by an agar plate dilution method.

Original languageEnglish
Pages (from-to)391-394
Number of pages4
JournalBiotechnology and Bioengineering
Issue number4
Publication statusPublished - 2001 Nov 8


  • Bactericide
  • Microbial chip
  • Respiration activity
  • Scanning electrochemical microscopy (SECM)


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