A microbial chip for glucose sensing studied with scanning electrochemical microscopy (SECM)

Takatoshi Kaya; Kuniaki Nagamine; Daisuke Oyamatsu; Matsuhiko Nishizawa; Tomokazu Matsue

doi:10.5796/electrochemistry.71.436

A microbial chip for glucose sensing studied with scanning electrochemical microscopy (SECM)

Takatoshi Kaya, Kuniaki Nagamine, Daisuke Oyamatsu, Matsuhiko Nishizawa, Tomokazu Matsue

Tohoku University

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

A microbial chip for bioassay was fabricated and its performance was characterized by scanning electrochemical microscopy (SECM). The microbial chip was fabricated by filling the micropores on a glass substrate with collagen gel embedding Escherichia coli (E.coli) cells. For measuring the activity of E.coli cells, the SECM measurement using ferricyanide as an electron mediator showed that the respiration activity of the immobilized E.coli cells increased with the concentration of glucose in the solution. The results suggested that the microbial chip can be used as a chemical sensor for nutritive species.

Original language	English
Pages (from-to)	436-438
Number of pages	3
Journal	Electrochemistry
Volume	71
Issue number	6
DOIs	https://doi.org/10.5796/electrochemistry.71.436
Publication status	Published - 2003 Jun

Keywords

Collagen Gel Microstructure
Glucose Sensing
Microbial Chip
Scanning Electrochemical Microscopy

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abstract = "A microbial chip for bioassay was fabricated and its performance was characterized by scanning electrochemical microscopy (SECM). The microbial chip was fabricated by filling the micropores on a glass substrate with collagen gel embedding Escherichia coli (E.coli) cells. For measuring the activity of E.coli cells, the SECM measurement using ferricyanide as an electron mediator showed that the respiration activity of the immobilized E.coli cells increased with the concentration of glucose in the solution. The results suggested that the microbial chip can be used as a chemical sensor for nutritive species.",

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author = "Takatoshi Kaya and Kuniaki Nagamine and Daisuke Oyamatsu and Matsuhiko Nishizawa and Tomokazu Matsue",

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AU - Nagamine, Kuniaki

AU - Oyamatsu, Daisuke

AU - Nishizawa, Matsuhiko

AU - Matsue, Tomokazu

PY - 2003/6

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N2 - A microbial chip for bioassay was fabricated and its performance was characterized by scanning electrochemical microscopy (SECM). The microbial chip was fabricated by filling the micropores on a glass substrate with collagen gel embedding Escherichia coli (E.coli) cells. For measuring the activity of E.coli cells, the SECM measurement using ferricyanide as an electron mediator showed that the respiration activity of the immobilized E.coli cells increased with the concentration of glucose in the solution. The results suggested that the microbial chip can be used as a chemical sensor for nutritive species.

AB - A microbial chip for bioassay was fabricated and its performance was characterized by scanning electrochemical microscopy (SECM). The microbial chip was fabricated by filling the micropores on a glass substrate with collagen gel embedding Escherichia coli (E.coli) cells. For measuring the activity of E.coli cells, the SECM measurement using ferricyanide as an electron mediator showed that the respiration activity of the immobilized E.coli cells increased with the concentration of glucose in the solution. The results suggested that the microbial chip can be used as a chemical sensor for nutritive species.

KW - Collagen Gel Microstructure

KW - Glucose Sensing

KW - Microbial Chip

KW - Scanning Electrochemical Microscopy

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A microbial chip for glucose sensing studied with scanning electrochemical microscopy (SECM)

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Keywords

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