A millisecond structured illumination microscope for super-resolution live cell imaging

Tomu Suzuki; Shinji Kajimoto; Narufumi Kitamura; Mayumi Takano-Kasuya; Naoko Furusawa; Yasushi Nakano; Hiroshi Fukumura; Kohsuke Gonda; Takakazu Nakabayashi

doi:10.35848/1882-0786/ab7cef

A millisecond structured illumination microscope for super-resolution live cell imaging

Tomu Suzuki, Shinji Kajimoto, Narufumi Kitamura, Mayumi Takano-Kasuya, Naoko Furusawa, Yasushi Nakano, Hiroshi Fukumura, Kohsuke Gonda, Takakazu Nakabayashi

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

In typical super-resolution microscopy, high temporal resolution is sacrificed to achieve a spatial resolution beyond the diffraction limit. In this study, we have developed a new structured illumination microscopy (SIM) system using two Pockels cells, achieving a temporal resolution of 1 ms. The introduction of the two Pockels cells enables control of the phases of the illumination pattern along the X- A nd Y-axes in microseconds. We obtained five images consecutively with different illumination patterns and reconstructed a super-resolution image. We observed fluorescent nanoparticles in living cells for 8 s with super resolution and a frame rate of 1,000 fps.

Original language	English
Article number	045002
Journal	Applied Physics Express
Volume	13
Issue number	4
DOIs	https://doi.org/10.35848/1882-0786/ab7cef
Publication status	Published - 2020 Apr 1

ASJC Scopus subject areas

Engineering(all)
Physics and Astronomy(all)

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title = "A millisecond structured illumination microscope for super-resolution live cell imaging",

abstract = "In typical super-resolution microscopy, high temporal resolution is sacrificed to achieve a spatial resolution beyond the diffraction limit. In this study, we have developed a new structured illumination microscopy (SIM) system using two Pockels cells, achieving a temporal resolution of 1 ms. The introduction of the two Pockels cells enables control of the phases of the illumination pattern along the X- A nd Y-axes in microseconds. We obtained five images consecutively with different illumination patterns and reconstructed a super-resolution image. We observed fluorescent nanoparticles in living cells for 8 s with super resolution and a frame rate of 1,000 fps.",

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AU - Suzuki, Tomu

AU - Kajimoto, Shinji

AU - Kitamura, Narufumi

AU - Takano-Kasuya, Mayumi

AU - Furusawa, Naoko

AU - Nakano, Yasushi

AU - Fukumura, Hiroshi

AU - Gonda, Kohsuke

AU - Nakabayashi, Takakazu

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N2 - In typical super-resolution microscopy, high temporal resolution is sacrificed to achieve a spatial resolution beyond the diffraction limit. In this study, we have developed a new structured illumination microscopy (SIM) system using two Pockels cells, achieving a temporal resolution of 1 ms. The introduction of the two Pockels cells enables control of the phases of the illumination pattern along the X- A nd Y-axes in microseconds. We obtained five images consecutively with different illumination patterns and reconstructed a super-resolution image. We observed fluorescent nanoparticles in living cells for 8 s with super resolution and a frame rate of 1,000 fps.

AB - In typical super-resolution microscopy, high temporal resolution is sacrificed to achieve a spatial resolution beyond the diffraction limit. In this study, we have developed a new structured illumination microscopy (SIM) system using two Pockels cells, achieving a temporal resolution of 1 ms. The introduction of the two Pockels cells enables control of the phases of the illumination pattern along the X- A nd Y-axes in microseconds. We obtained five images consecutively with different illumination patterns and reconstructed a super-resolution image. We observed fluorescent nanoparticles in living cells for 8 s with super resolution and a frame rate of 1,000 fps.

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A millisecond structured illumination microscope for super-resolution live cell imaging

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