A modified bait and switch strategy for the generation of esterolytic abzymes using concerted catalytic mechanisms

Yoshiharu Iwabuchi; Shinwa Kurihara; Motoko Oda; Ikuo Fujii

doi:10.1016/S0040-4039(99)00972-7

A modified bait and switch strategy for the generation of esterolytic abzymes using concerted catalytic mechanisms

Yoshiharu Iwabuchi, Shinwa Kurihara, Motoko Oda, Ikuo Fujii

PHA - Molecular Pharmaceutical Science

Biomolecular Engineering Research Institute

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Esterolytic abzymes were generated via immunization with a haptenic diphosphonate mounted with two distinct traps actuated by the 'bait and switch' and 'transition-state mimic' concepts. One monoclonal antibody, 12F12, was characterized in detail, and showed significant rate enhancement and unique substrate specificity. Experimental data support that the antibody catalyzes the reactions with a concerted catalytic mechanism.

Original language	English
Pages (from-to)	5341-5344
Number of pages	4
Journal	Tetrahedron Letters
Volume	40
Issue number	29
DOIs	https://doi.org/10.1016/S0040-4039(99)00972-7
Publication status	Published - 1999 Jul 16

Keywords

Amino acids and derivatives
Antibodies
Catalysis
Ester hydrolysis

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10.1016/S0040-4039(99)00972-7

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T1 - A modified bait and switch strategy for the generation of esterolytic abzymes using concerted catalytic mechanisms

AU - Iwabuchi, Yoshiharu

AU - Kurihara, Shinwa

AU - Oda, Motoko

AU - Fujii, Ikuo

PY - 1999/7/16

Y1 - 1999/7/16

N2 - Esterolytic abzymes were generated via immunization with a haptenic diphosphonate mounted with two distinct traps actuated by the 'bait and switch' and 'transition-state mimic' concepts. One monoclonal antibody, 12F12, was characterized in detail, and showed significant rate enhancement and unique substrate specificity. Experimental data support that the antibody catalyzes the reactions with a concerted catalytic mechanism.

AB - Esterolytic abzymes were generated via immunization with a haptenic diphosphonate mounted with two distinct traps actuated by the 'bait and switch' and 'transition-state mimic' concepts. One monoclonal antibody, 12F12, was characterized in detail, and showed significant rate enhancement and unique substrate specificity. Experimental data support that the antibody catalyzes the reactions with a concerted catalytic mechanism.

KW - Amino acids and derivatives

KW - Antibodies

KW - Catalysis

KW - Ester hydrolysis

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JO - Tetrahedron Letters

JF - Tetrahedron Letters

IS - 29

ER -

A modified bait and switch strategy for the generation of esterolytic abzymes using concerted catalytic mechanisms

Abstract

Keywords

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