Esterolytic abzymes were generated via immunization with a haptenic diphosphonate mounted with two distinct traps actuated by the 'bait and switch' and 'transition-state mimic' concepts. One monoclonal antibody, 12F12, was characterized in detail, and showed significant rate enhancement and unique substrate specificity. Experimental data support that the antibody catalyzes the reactions with a concerted catalytic mechanism.
- Amino acids and derivatives
- Ester hydrolysis