A nanocluster design for the construction of artificial cellulosomes

Do Myoung Kim; Hikaru Nakazawa; Mitsuo Umetsu; Takashi Matsuyama; Nobuhiro Ishida; Akinori Ikeuchi; Haruo Takahashi; Ryutaro Asano; Izumi Kumagai

doi:10.1039/c2cy00371f

A nanocluster design for the construction of artificial cellulosomes

Do Myoung Kim, Hikaru Nakazawa, Mitsuo Umetsu, Takashi Matsuyama, Nobuhiro Ishida, Akinori Ikeuchi, Haruo Takahashi, Ryutaro Asano, Izumi Kumagai

ENG - Biomolecular Engineering

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

We describe here the construction of artificial cellulosomes by nanoclustering recombinant cellulolytic modules on non-cellulosome-derived scaffolds. Catalytic and cellulose-binding domain modules derived from cellulosomes were assembled on streptavidin and on inorganic nanoparticles. Heteroclustering of the modules significantly promoted the activity of the assembled catalytic modules for degradation of water-insoluble substrates.

Original language	English
Pages (from-to)	499-503
Number of pages	5
Journal	Catalysis Science and Technology
Volume	2
Issue number	3
DOIs	https://doi.org/10.1039/c2cy00371f
Publication status	Published - 2012 Mar

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AU - Nakazawa, Hikaru

AU - Umetsu, Mitsuo

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AU - Ishida, Nobuhiro

AU - Ikeuchi, Akinori

AU - Takahashi, Haruo

AU - Asano, Ryutaro

AU - Kumagai, Izumi

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AB - We describe here the construction of artificial cellulosomes by nanoclustering recombinant cellulolytic modules on non-cellulosome-derived scaffolds. Catalytic and cellulose-binding domain modules derived from cellulosomes were assembled on streptavidin and on inorganic nanoparticles. Heteroclustering of the modules significantly promoted the activity of the assembled catalytic modules for degradation of water-insoluble substrates.

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A nanocluster design for the construction of artificial cellulosomes

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