TY - JOUR
T1 - A new endogenous opioid peptide from bovine adrenal medulla
T2 - Isolation and amino acid sequence of a dodecapeptide (BAM-12P)
AU - Mizuno, Kensaku
AU - Minamino, Naoto
AU - Kangawa, Kenji
AU - Matsuo, Hisayuki
PY - 1980/1/1
Y1 - 1980/1/1
N2 - A new dodecapeptide, designated BAM-12P, which released an N-terminal [Arg6]-Met-enkephalin by trypsinization, has been isolated from bovine adrenal medulla in a pure state by the aid of a newly developed radioimmunoassay utilizing antiserum against [Arg6]-Leu-enkephalin, which shows 5% cross-reactivity with [Arg6]-Met-enkephalin. Amino acid sequence of the peptide has been established to be: Tyr-Gly-Gly-Phe-Met-Arg-Arg-Val-Gly-Arg-Pro-Glu. On the basis of its structure, the dodecapeptide is considered to be a novel pro-Met-enkephalin, unrelated to β-lipotropin.
AB - A new dodecapeptide, designated BAM-12P, which released an N-terminal [Arg6]-Met-enkephalin by trypsinization, has been isolated from bovine adrenal medulla in a pure state by the aid of a newly developed radioimmunoassay utilizing antiserum against [Arg6]-Leu-enkephalin, which shows 5% cross-reactivity with [Arg6]-Met-enkephalin. Amino acid sequence of the peptide has been established to be: Tyr-Gly-Gly-Phe-Met-Arg-Arg-Val-Gly-Arg-Pro-Glu. On the basis of its structure, the dodecapeptide is considered to be a novel pro-Met-enkephalin, unrelated to β-lipotropin.
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U2 - 10.1016/S0006-291X(80)80064-7
DO - 10.1016/S0006-291X(80)80064-7
M3 - Article
C2 - 7417331
AN - SCOPUS:0019221013
VL - 95
SP - 1482
EP - 1488
JO - Topics in Catalysis
JF - Topics in Catalysis
IS - 4
ER -