A new low density lipoprotein receptor related protein, LRP5, is expressed in hepatocytes and adrenal cortex, and recognizes apolipoprotein E

Dong Ho Kim; Yousuke Inagaki; Takashi Suzuki; Ryoichi X. Ioka; Shiroh Z. Yoshioka; Kenta Magoori; Man Jong Kang; Yuko Cho; Akira Z. Nakano; Qiong Liu; Takahiro Fujino; Hiroyuki Suzuki; Hironobu Sasano; Tokuo T. Yamamoto

doi:10.1093/oxfordjournals.jbchem.a022223

A new low density lipoprotein receptor related protein, LRP5, is expressed in hepatocytes and adrenal cortex, and recognizes apolipoprotein E

Dong Ho Kim, Yousuke Inagaki, Takashi Suzuki, Ryoichi X. Ioka, Shiroh Z. Yoshioka, Kenta Magoori, Man Jong Kang, Yuko Cho, Akira Z. Nakano, Qiong Liu, Takahiro Fujino, Hiroyuki Suzuki, Hironobu Sasano, Tokuo T. Yamamoto

Tohoku University

Research output: Contribution to journal › Article › peer-review

104 Citations (Scopus)

Abstract

The isolation and characterization of rabbit and human cDNAs revealed a new low density lipoprotein receptor (LDLR)-related protein (LRP) designated as LRP5. Human LRP5 cDNA encodes a 1616-amino acid type I membrane-like protein with three ligand binding repeats in its extracellular region. LDLR-deficient cells transduced by recombinant adenovirus containing human LRP5 exhibited increased binding of apolipoprotein E (apoE)-enriched β-migrating very low density lipoprotein. Northern blotting and in situ hybridization revealed a high level of LRP5 expression in hepatocytes and the adrenal gland cortex. In LDLR-deficient Watanabe heritable hyperlipidemic rabbits, LRP5 mRNA was increased in the liver and accumulated in cholesterol-laden foam cells of atherosclerotic lesions.

Original language	English
Pages (from-to)	1072-1076
Number of pages	5
Journal	Journal of Biochemistry
Volume	124
Issue number	6
DOIs	https://doi.org/10.1093/oxfordjournals.jbchem.a022223
Publication status	Published - 1998

Keywords

apoE
LDL receptor family
LDL receptor-related protein
Lipoprotein

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10.1093/oxfordjournals.jbchem.a022223

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Kim, D. H., Inagaki, Y., Suzuki, T., Ioka, R. X., Yoshioka, S. Z., Magoori, K., Kang, M. J., Cho, Y., Nakano, A. Z., Liu, Q., Fujino, T., Suzuki, H., Sasano, H., & Yamamoto, T. T. (1998). A new low density lipoprotein receptor related protein, LRP5, is expressed in hepatocytes and adrenal cortex, and recognizes apolipoprotein E. Journal of Biochemistry, 124(6), 1072-1076. https://doi.org/10.1093/oxfordjournals.jbchem.a022223

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title = "A new low density lipoprotein receptor related protein, LRP5, is expressed in hepatocytes and adrenal cortex, and recognizes apolipoprotein E",

abstract = "The isolation and characterization of rabbit and human cDNAs revealed a new low density lipoprotein receptor (LDLR)-related protein (LRP) designated as LRP5. Human LRP5 cDNA encodes a 1616-amino acid type I membrane-like protein with three ligand binding repeats in its extracellular region. LDLR-deficient cells transduced by recombinant adenovirus containing human LRP5 exhibited increased binding of apolipoprotein E (apoE)-enriched β-migrating very low density lipoprotein. Northern blotting and in situ hybridization revealed a high level of LRP5 expression in hepatocytes and the adrenal gland cortex. In LDLR-deficient Watanabe heritable hyperlipidemic rabbits, LRP5 mRNA was increased in the liver and accumulated in cholesterol-laden foam cells of atherosclerotic lesions.",

keywords = "apoE, LDL receptor family, LDL receptor-related protein, Lipoprotein",

author = "Kim, {Dong Ho} and Yousuke Inagaki and Takashi Suzuki and Ioka, {Ryoichi X.} and Yoshioka, {Shiroh Z.} and Kenta Magoori and Kang, {Man Jong} and Yuko Cho and Nakano, {Akira Z.} and Qiong Liu and Takahiro Fujino and Hiroyuki Suzuki and Hironobu Sasano and Yamamoto, {Tokuo T.}",

year = "1998",

doi = "10.1093/oxfordjournals.jbchem.a022223",

language = "English",

volume = "124",

pages = "1072--1076",

journal = "Journal of Biochemistry",

issn = "0021-924X",

publisher = "Oxford University Press",

number = "6",

}

Kim, DH, Inagaki, Y, Suzuki, T, Ioka, RX, Yoshioka, SZ, Magoori, K, Kang, MJ, Cho, Y, Nakano, AZ, Liu, Q, Fujino, T, Suzuki, H, Sasano, H & Yamamoto, TT 1998, 'A new low density lipoprotein receptor related protein, LRP5, is expressed in hepatocytes and adrenal cortex, and recognizes apolipoprotein E', Journal of Biochemistry, vol. 124, no. 6, pp. 1072-1076. https://doi.org/10.1093/oxfordjournals.jbchem.a022223

TY - JOUR

T1 - A new low density lipoprotein receptor related protein, LRP5, is expressed in hepatocytes and adrenal cortex, and recognizes apolipoprotein E

AU - Kim, Dong Ho

AU - Inagaki, Yousuke

AU - Suzuki, Takashi

AU - Ioka, Ryoichi X.

AU - Yoshioka, Shiroh Z.

AU - Magoori, Kenta

AU - Kang, Man Jong

AU - Cho, Yuko

AU - Nakano, Akira Z.

AU - Liu, Qiong

AU - Fujino, Takahiro

AU - Suzuki, Hiroyuki

AU - Sasano, Hironobu

AU - Yamamoto, Tokuo T.

PY - 1998

Y1 - 1998

N2 - The isolation and characterization of rabbit and human cDNAs revealed a new low density lipoprotein receptor (LDLR)-related protein (LRP) designated as LRP5. Human LRP5 cDNA encodes a 1616-amino acid type I membrane-like protein with three ligand binding repeats in its extracellular region. LDLR-deficient cells transduced by recombinant adenovirus containing human LRP5 exhibited increased binding of apolipoprotein E (apoE)-enriched β-migrating very low density lipoprotein. Northern blotting and in situ hybridization revealed a high level of LRP5 expression in hepatocytes and the adrenal gland cortex. In LDLR-deficient Watanabe heritable hyperlipidemic rabbits, LRP5 mRNA was increased in the liver and accumulated in cholesterol-laden foam cells of atherosclerotic lesions.

AB - The isolation and characterization of rabbit and human cDNAs revealed a new low density lipoprotein receptor (LDLR)-related protein (LRP) designated as LRP5. Human LRP5 cDNA encodes a 1616-amino acid type I membrane-like protein with three ligand binding repeats in its extracellular region. LDLR-deficient cells transduced by recombinant adenovirus containing human LRP5 exhibited increased binding of apolipoprotein E (apoE)-enriched β-migrating very low density lipoprotein. Northern blotting and in situ hybridization revealed a high level of LRP5 expression in hepatocytes and the adrenal gland cortex. In LDLR-deficient Watanabe heritable hyperlipidemic rabbits, LRP5 mRNA was increased in the liver and accumulated in cholesterol-laden foam cells of atherosclerotic lesions.

KW - apoE

KW - LDL receptor family

KW - LDL receptor-related protein

KW - Lipoprotein

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U2 - 10.1093/oxfordjournals.jbchem.a022223

DO - 10.1093/oxfordjournals.jbchem.a022223

M3 - Article

AN - SCOPUS:0032446373

SN - 0021-924X

VL - 124

SP - 1072

EP - 1076

JO - Journal of Biochemistry

JF - Journal of Biochemistry

IS - 6

ER -

A new low density lipoprotein receptor related protein, LRP5, is expressed in hepatocytes and adrenal cortex, and recognizes apolipoprotein E

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