A new primer set to amplify the mitochondrial cytochrome C oxidase subunit I (COI) gene in the DHA-rich microalgae, the genus Aurantiochytrium

Goh Nishitani; Masaki Yoshida

doi:10.1264/jsme2.ME17145

A new primer set to amplify the mitochondrial cytochrome C oxidase subunit I (COI) gene in the DHA-rich microalgae, the genus Aurantiochytrium

Goh Nishitani, Masaki Yoshida

AGR - Agricultural Bioscience

University of Tsukuba

Research output: Contribution to journal › Article › peer-review

Abstract

This study was performed in order to develop a primer set for mitochondrial cytochrome c oxidase subunit I (COI) in the DHA-rich microalgae of the genus Aurantiochytrium. The performance of the primer set was tested using 12 Aurantiochytrium strains and other thraustochytrid species. There were no genetic polymorphisms in the mitochondrial sequences from the Aurantiochytrium strains, in contrast to the nuclear 18S rRNA gene sequence. This newly developed primer set amplified sequences from Aurantiochytrium and closely related genera, and may be useful for species identification and clarifying the genetic diversity of Aurantiochytrium in the field.

Original language	English
Pages (from-to)	227-229
Number of pages	3
Journal	Microbes and Environments
Volume	33
Issue number	2
DOIs	https://doi.org/10.1264/jsme2.ME17145
Publication status	Published - 2018

Keywords

Aurantiochytrium
COI
Mitochondrial primer

Access to Document

10.1264/jsme2.ME17145

Cite this

@article{4c0d366654ee466d9da3e138b69373b5,

title = "A new primer set to amplify the mitochondrial cytochrome C oxidase subunit I (COI) gene in the DHA-rich microalgae, the genus Aurantiochytrium",

abstract = "This study was performed in order to develop a primer set for mitochondrial cytochrome c oxidase subunit I (COI) in the DHA-rich microalgae of the genus Aurantiochytrium. The performance of the primer set was tested using 12 Aurantiochytrium strains and other thraustochytrid species. There were no genetic polymorphisms in the mitochondrial sequences from the Aurantiochytrium strains, in contrast to the nuclear 18S rRNA gene sequence. This newly developed primer set amplified sequences from Aurantiochytrium and closely related genera, and may be useful for species identification and clarifying the genetic diversity of Aurantiochytrium in the field.",

keywords = "Aurantiochytrium, COI, Mitochondrial primer",

author = "Goh Nishitani and Masaki Yoshida",

note = "Funding Information: Thanks are due to Dr. Makoto M. Watanabe and Dr. Iwane Suzuki of the University of Tsukuba for their kind support. This research was supported by funding from Hashimoto-road Co., Ltd., Higashimatsushima-shi, Miyagi Prefecture, Japan. Publisher Copyright: {\textcopyright} 2018, Japanese Society of Microbial Ecology. All rights reserved.",

year = "2018",

doi = "10.1264/jsme2.ME17145",

language = "English",

volume = "33",

pages = "227--229",

journal = "Microbes and Environments",

issn = "1342-6311",

publisher = "Japanese Society Of Microbial Ecology",

number = "2",

}

TY - JOUR

T1 - A new primer set to amplify the mitochondrial cytochrome C oxidase subunit I (COI) gene in the DHA-rich microalgae, the genus Aurantiochytrium

AU - Nishitani, Goh

AU - Yoshida, Masaki

N1 - Funding Information: Thanks are due to Dr. Makoto M. Watanabe and Dr. Iwane Suzuki of the University of Tsukuba for their kind support. This research was supported by funding from Hashimoto-road Co., Ltd., Higashimatsushima-shi, Miyagi Prefecture, Japan. Publisher Copyright: © 2018, Japanese Society of Microbial Ecology. All rights reserved.

PY - 2018

Y1 - 2018

N2 - This study was performed in order to develop a primer set for mitochondrial cytochrome c oxidase subunit I (COI) in the DHA-rich microalgae of the genus Aurantiochytrium. The performance of the primer set was tested using 12 Aurantiochytrium strains and other thraustochytrid species. There were no genetic polymorphisms in the mitochondrial sequences from the Aurantiochytrium strains, in contrast to the nuclear 18S rRNA gene sequence. This newly developed primer set amplified sequences from Aurantiochytrium and closely related genera, and may be useful for species identification and clarifying the genetic diversity of Aurantiochytrium in the field.

AB - This study was performed in order to develop a primer set for mitochondrial cytochrome c oxidase subunit I (COI) in the DHA-rich microalgae of the genus Aurantiochytrium. The performance of the primer set was tested using 12 Aurantiochytrium strains and other thraustochytrid species. There were no genetic polymorphisms in the mitochondrial sequences from the Aurantiochytrium strains, in contrast to the nuclear 18S rRNA gene sequence. This newly developed primer set amplified sequences from Aurantiochytrium and closely related genera, and may be useful for species identification and clarifying the genetic diversity of Aurantiochytrium in the field.

KW - Aurantiochytrium

KW - COI

KW - Mitochondrial primer

UR - http://www.scopus.com/inward/record.url?scp=85049634355&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85049634355&partnerID=8YFLogxK

U2 - 10.1264/jsme2.ME17145

DO - 10.1264/jsme2.ME17145

M3 - Article

C2 - 29863058

AN - SCOPUS:85049634355

SN - 1342-6311

VL - 33

SP - 227

EP - 229

JO - Microbes and Environments

JF - Microbes and Environments

IS - 2

ER -

A new primer set to amplify the mitochondrial cytochrome C oxidase subunit I (COI) gene in the DHA-rich microalgae, the genus Aurantiochytrium

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this