TY - JOUR
T1 - A novel application of nanohydroxyapatite/mesoporous silica biocomposite on treating dentin hypersensitivity
T2 - An in vitro study
AU - Yu, Jian
AU - Yang, Hongye
AU - Li, Kang
AU - Lei, Jinmei
AU - Zhou, Liqun
AU - Huang, Cui
N1 - Funding Information:
This work was financially supported by the National Natural Science Foundation of China (No. 81371191 ) and the Fundamental Research Funds for the Central Universities ( 2042015kf1052 ).
Publisher Copyright:
© 2016 Elsevier Ltd.
PY - 2016/7/1
Y1 - 2016/7/1
N2 - Objectives To fabricate a nanohydroxyapatite/mesoporous silica nanoparticle (nHAp@MSN) biocomposite and investigate its effectiveness on dentinal tubule occlusion, acid-resistant stability, and microtensile bond strength (MTBS). Methods The nHAp@MSN biocomposite was synthesized and characterized by X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy, N2 adsorption-desorption isotherms, field-emission scanning electron microscopy (FESEM), and high-resolution transmission electron microscopy (HRTEM). Thirty-two simulated sensitive dentin discs were prepared and randomly divided into four groups according to the following treatments (n = 8 each): Group 1, no treatment; Group 2, NovaMin, 15 s × 2; Group 3, MSN, 15 s × 2; Group 4, nHAp@MSN, 15 s × 2. Then, four discs per group were post-treated with 6 wt.% citric acid challenge to test their acid-resistant stability. The effects on dentinal tubule occlusion were observed by FESEM. A self-etch adhesive (G-Bond) was applied to evaluate the MTBS. The cytotoxicity was detected using the Cell Counting Kit-8 (CCK-8) assay. Results Results revealed that the nHAp@MSN biocomposite was successfully fabricated. nHAp@MSN could effectively occlude the dentinal tubules, and the intratubular crystals were tightly associated with the tubular wall. After citric acid attack, nHAp@MSN exhibited the highest acid-resistant stability among the four groups. Moreover, no significant difference in MTBS was noted among the four groups (P > 0.05). CCK-8 assay identified that nHAp@MSN induced no more than 20% cell death even at the highest concentration of 640 μg/mL. Conclusions The application of the nHAp@MSN biocomposite resulted in efficient dentinal tubule occlusion, acid-resistant stability, and did not compromise immediate bond strength between dentin and self-etch adhesive system. Clinical significance The nHAp@MSN biocomposite indicates enormous potential as a new strategy for relieving dentin hypersensitivity.
AB - Objectives To fabricate a nanohydroxyapatite/mesoporous silica nanoparticle (nHAp@MSN) biocomposite and investigate its effectiveness on dentinal tubule occlusion, acid-resistant stability, and microtensile bond strength (MTBS). Methods The nHAp@MSN biocomposite was synthesized and characterized by X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy, N2 adsorption-desorption isotherms, field-emission scanning electron microscopy (FESEM), and high-resolution transmission electron microscopy (HRTEM). Thirty-two simulated sensitive dentin discs were prepared and randomly divided into four groups according to the following treatments (n = 8 each): Group 1, no treatment; Group 2, NovaMin, 15 s × 2; Group 3, MSN, 15 s × 2; Group 4, nHAp@MSN, 15 s × 2. Then, four discs per group were post-treated with 6 wt.% citric acid challenge to test their acid-resistant stability. The effects on dentinal tubule occlusion were observed by FESEM. A self-etch adhesive (G-Bond) was applied to evaluate the MTBS. The cytotoxicity was detected using the Cell Counting Kit-8 (CCK-8) assay. Results Results revealed that the nHAp@MSN biocomposite was successfully fabricated. nHAp@MSN could effectively occlude the dentinal tubules, and the intratubular crystals were tightly associated with the tubular wall. After citric acid attack, nHAp@MSN exhibited the highest acid-resistant stability among the four groups. Moreover, no significant difference in MTBS was noted among the four groups (P > 0.05). CCK-8 assay identified that nHAp@MSN induced no more than 20% cell death even at the highest concentration of 640 μg/mL. Conclusions The application of the nHAp@MSN biocomposite resulted in efficient dentinal tubule occlusion, acid-resistant stability, and did not compromise immediate bond strength between dentin and self-etch adhesive system. Clinical significance The nHAp@MSN biocomposite indicates enormous potential as a new strategy for relieving dentin hypersensitivity.
KW - Dentin hypersensitivity
KW - Dentinal tubule
KW - Hydroxyapatite
KW - Mesoporous silica
KW - Microtensile bond strength
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U2 - 10.1016/j.jdent.2016.04.005
DO - 10.1016/j.jdent.2016.04.005
M3 - Article
C2 - 27101767
AN - SCOPUS:84969915616
SN - 0300-5712
VL - 50
SP - 21
EP - 29
JO - Journal of Dentistry
JF - Journal of Dentistry
ER -