@article{a1613617d4014c5d92d13d62e3828976,
title = "A novel motor, KIF13A, transports mannose-6-phosphate receptor to plasma membrane through direct interaction with AP-1 complex",
abstract = "Intracellular transport mediated by kinesin superfamily proteins (KIFs) is a highly regulated process. The molecular mechanism of KIFs binding to their respective cargoes remains unclear. We report that KIF13A is a novel plus end-directed microtubule-dependent motor protein and associates with β1-adaptin, a subunit of the AP-1 adaptor complex. The cargo vesicles of KIF13A contained AP-1 and mannnose-6-phosphate receptor (M6PR). Overexpression of KIF13A resulted in mislocalization of the AP-1 and the M6PR. Functional blockade of KIF13A reduced cell surface expression of the M6PR. Thus, KIF13A transports M6PR-containing vesicles and targets the M6PR from TGN to the plasma membrane via direct interaction with the AP-1 adaptor complex.",
author = "Terunaga Nakagawa and Mitsutoshi Setou and Seog, {Dae Hyun} and Kouetsu Ogasawara and Naoshi Dohmae and Koji Takio and Nobutaka Hirokawa",
note = "Funding Information: We are grateful to the following: E. Rodriguez-Boulan (Cornell University Medical College) for MDCKII cells; Shigekazu Nagata (Osaka University, Japan) for the pBOSS vector; Kazuhisa Nakayama (Tsukuba University, Japan) for human β1- and β2-adaptin cDNA; William J. Brown (Cornell University) for anti-M6PR; Linton Traub (Washington University, St. Louis) for anti-μ1A-adaptin; Kazumi Ishidoh (Juntendo University, Japan) for anti-cathepsin D; and Tadatsugu Taniguchi (University of Tokyo, Japan) for making the FACScalibur (Becton Dickinson) available. We thank Masae Sugaya, Hiromi Sato, and Haruyo Fukuda for secretarial and technical assistance. This work was supported by COE grants from the Ministry of Education, Science, Sports and Culture of Japan to N. H.; T. N. and D.-H. S. were Research Fellows of the Japan Society for Promotion of Science. ",
year = "2000",
doi = "10.1016/S0092-8674(00)00161-6",
language = "English",
volume = "103",
pages = "569--581",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "4",
}