TY - JOUR
T1 - A novel non-thermostable deuterolysin from Aspergillus oryzae
AU - Maeda, Hiroshi
AU - Katase, Toru
AU - Sakai, Daisuke
AU - Takeuchi, Michio
AU - Kusumoto, Ken Ichi
AU - Amano, Hitoshi
AU - Ishida, Hiroki
AU - Abe, Keietsu
AU - Yamagata, Youhei
N1 - Publisher Copyright:
© 2016 Japan Society for Bioscience, Biotechnology, and Agrochemistry.
PY - 2016
Y1 - 2016
N2 - Three putative deuterolysin (EC 3.4.24.29) genes (deuA, deuB, and deuC) were found in the Aspergillus oryzae genome database (http://www.bio.nite.go.jp/dogan/project/view/AO). One of these genes, deuA, was corresponding to NpII gene, previously reported. DeuA and DeuB were overexpressed by recombinant A. oryzae and were purified. The degradation profiles against protein substrates of both enzymes were similar, but DeuB showed wider substrate specificity against peptidyl MCA-substrates compared with DeuA. Enzymatic profiles of DeuB except for thermostability also resembled those of DeuA. DeuB was inactivated by heat treatment above 80° C, different from thermostable DeuA. Transcription analysis in wild type A. oryzae showed only deuB was expressed in liquid culture, and the addition of the proteinous substrate upregulated the transcription. Furthermore, the NaNO3 addition seems to eliminate the effect of proteinous substrate for the transcription of deuB.
AB - Three putative deuterolysin (EC 3.4.24.29) genes (deuA, deuB, and deuC) were found in the Aspergillus oryzae genome database (http://www.bio.nite.go.jp/dogan/project/view/AO). One of these genes, deuA, was corresponding to NpII gene, previously reported. DeuA and DeuB were overexpressed by recombinant A. oryzae and were purified. The degradation profiles against protein substrates of both enzymes were similar, but DeuB showed wider substrate specificity against peptidyl MCA-substrates compared with DeuA. Enzymatic profiles of DeuB except for thermostability also resembled those of DeuA. DeuB was inactivated by heat treatment above 80° C, different from thermostable DeuA. Transcription analysis in wild type A. oryzae showed only deuB was expressed in liquid culture, and the addition of the proteinous substrate upregulated the transcription. Furthermore, the NaNO3 addition seems to eliminate the effect of proteinous substrate for the transcription of deuB.
KW - Aspergillus oryzae
KW - Deuterolysin
KW - Expression specificity
KW - Thermostability
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U2 - 10.1080/09168451.2016.1166933
DO - 10.1080/09168451.2016.1166933
M3 - Article
C2 - 27050120
AN - SCOPUS:84984670071
SN - 0916-8451
VL - 80
SP - 1813
EP - 1819
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
IS - 9
ER -