TY - JOUR
T1 - A Novel Predictive Method for Assessing the Quality of Isolated Pancreatic Islets Using Scanning Electrochemical Microscopy
AU - Goto, M.
AU - Abe, H.
AU - Ito-Sasaki, T.
AU - Goto, M.
AU - Inagaki, A.
AU - Ogawa, N.
AU - Fujimori, K.
AU - Kurokawa, Y.
AU - Matsue, T.
AU - Satomi, S.
N1 - Funding Information:
This study has been supported by grants from Innovation Plaza Miyagi of JST (Japan Science and Technology Agency) and the Japanese Grant-in-Aid for Scientific Research (A).
PY - 2009/1
Y1 - 2009/1
N2 - Introduction: The current methods for evaluating islet potency are not useful in clinical transplantation. Therefore, we need reliable, rapid methods enabling accurate prediction of islet quality. Materials and Methods: We evaluated respiratory activity using scanning electrochemical microscopy (SECM), glucose-stimulated respiratory activity, glucose-stimulated insulin release, ADP/ATP assays, insulin/DNA levels, and Trypan blue exclusion tests as predictive methods for the ability of isolated rat islets to cure syngeneic diabetic rats. Results: Although glucose-stimulated respiratory activity, basal respiratory activity, ADP/ATP ratio, and glucose-stimulated insulin release were significantly correlated with the outcome of transplantation into diabetic rats, there was no correlation between outcomes, insulin/DNA ratios, and Trypan blue exclusion tests. The glucose-stimulated respiratory activity in islet preparations that could cure diabetic rats was significantly greater than those unable to cure diabetes. Rat islets with >1.5-fold glucose-stimulated respiratory activity consistently cured diabetic rats, whereas those with a value <1.5 hardly cured any rats. Conclusion: Measurement of the glucose-stimulated respiratory activity using SECM technique is a novel method that may be useful as a rapid, potent predictor of the outcome of clinical islet transplantation.
AB - Introduction: The current methods for evaluating islet potency are not useful in clinical transplantation. Therefore, we need reliable, rapid methods enabling accurate prediction of islet quality. Materials and Methods: We evaluated respiratory activity using scanning electrochemical microscopy (SECM), glucose-stimulated respiratory activity, glucose-stimulated insulin release, ADP/ATP assays, insulin/DNA levels, and Trypan blue exclusion tests as predictive methods for the ability of isolated rat islets to cure syngeneic diabetic rats. Results: Although glucose-stimulated respiratory activity, basal respiratory activity, ADP/ATP ratio, and glucose-stimulated insulin release were significantly correlated with the outcome of transplantation into diabetic rats, there was no correlation between outcomes, insulin/DNA ratios, and Trypan blue exclusion tests. The glucose-stimulated respiratory activity in islet preparations that could cure diabetic rats was significantly greater than those unable to cure diabetes. Rat islets with >1.5-fold glucose-stimulated respiratory activity consistently cured diabetic rats, whereas those with a value <1.5 hardly cured any rats. Conclusion: Measurement of the glucose-stimulated respiratory activity using SECM technique is a novel method that may be useful as a rapid, potent predictor of the outcome of clinical islet transplantation.
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U2 - 10.1016/j.transproceed.2008.10.075
DO - 10.1016/j.transproceed.2008.10.075
M3 - Article
C2 - 19249542
AN - SCOPUS:60649086201
SN - 0041-1345
VL - 41
SP - 311
EP - 313
JO - Transplantation Proceedings
JF - Transplantation Proceedings
IS - 1
ER -
