TY - JOUR
T1 - A Novel Somatic Point Mutation of the RET Proto‐oncogene in Tumor Tissues of Small Cell Lung Cancer Patients
AU - Futami, Hitoyasu
AU - Egawa, Shin‐ichi ‐i
AU - Tsukada, Toshihiko
AU - Maruyama, Kouji
AU - Bandoh, Shuji
AU - Noguchi, Masayuki
AU - Yamaguchi, Ken
PY - 1995/12
Y1 - 1995/12
N2 - We examined whether the novel point mutation from GCC (Ala) to GAC (Asp) at codon 664 in exon 11 of RET proto‐oncogene, which we had found in two small cell lung carcinoma (SCLC) cell lines, existed in genomic DNA of tumor tissues of the two SCLC patients from whom these SCLC cell lines were derived. Sequence analysis revealed that point mutation identical to that of the SCLC cell lines was present in amplified alleles of single‐strand conformational variants in genomic DNA of the tumor tissues, whereas it was not detected in genomic DNA of non‐tumor tissues of the patients. These results indicate that this mutation had initially occurred in the SCLC patients and was of somatic origin.
AB - We examined whether the novel point mutation from GCC (Ala) to GAC (Asp) at codon 664 in exon 11 of RET proto‐oncogene, which we had found in two small cell lung carcinoma (SCLC) cell lines, existed in genomic DNA of tumor tissues of the two SCLC patients from whom these SCLC cell lines were derived. Sequence analysis revealed that point mutation identical to that of the SCLC cell lines was present in amplified alleles of single‐strand conformational variants in genomic DNA of the tumor tissues, whereas it was not detected in genomic DNA of non‐tumor tissues of the patients. These results indicate that this mutation had initially occurred in the SCLC patients and was of somatic origin.
KW - Medullary thyroid carcinoma
KW - Multiple endocrine neoplasia
KW - Point mutation
KW - RET
KW - Small cell lung carcinoma
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U2 - 10.1111/j.1349-7006.1995.tb03304.x
DO - 10.1111/j.1349-7006.1995.tb03304.x
M3 - Article
C2 - 8635999
AN - SCOPUS:84989423207
SN - 0910-5050
VL - 86
SP - 1127
EP - 1130
JO - Japanese Journal of Cancer Research
JF - Japanese Journal of Cancer Research
IS - 12
ER -
