TY - JOUR
T1 - A Novel Tool for Detecting Amyloid Deposits in Systemic Amyloidosis in Vitro and in Vivo
AU - Ando, Yukio
AU - Haraoka, Katsuki
AU - Terazaki, Hisayasu
AU - Tanoue, Yutaka
AU - Ishikawa, Kensuke
AU - Katsuragi, Shoichi
AU - Nakamura, Masaaki
AU - Sun, Xuguo
AU - Nakagawa, Kazuko
AU - Sasamoto, Kazumi
AU - Takesako, Kazuhiro
AU - Ishizaki, Takashi
AU - Sasaki, Yutaka
AU - Doh-Ura, Katsumi
N1 - Funding Information:
YA and KH contributed equally to the study. This work was supported by grants from the Amyloidosis Research Committee and the Pathogenesis, Therapy of Hereditary Neuropathy Research Committee, Surveys and Research on Specific Disease, the Ministry of Health and Welfare of Japan, Charitable Trust Clinical Pathology Research Foundation of Japan, and Grants-in-Aid for Scientific Research (C) 13670655 and (B) 15390275 from the Ministry of Education, Science, Sports and Culture of Japan. Address reprint requests to: Dr. Y. Ando, Department of Laboratory Medicine, Kumamoto University School of Medicine, Japan.
PY - 2003/12
Y1 - 2003/12
N2 - We synthesized (trans, trans)-1-bromo-2,5-bis-(3-hydroxycarbonyl-4-hydroxy)styrylbenzene (BSB) and used this compound to detect amyloid fibrils in autopsy and biopsy samples from patients with localized amyloidosis, such as familial prion disease, and systemic amyloidosis, such as familial amyloidotic polyneuropathy, amyloid A (AA) amyloidosis, light chain (AL) amyloidosis, and dialysis-related amyloidosis. BSB showed reactions in all Congo red-positive and immunoreactive regions of the samples examined in the study, and some amyloid fibrils in the tissues could be detected more precisely with BSB than with the other methods. In the mouse model of AA amyloidosis, injected BSB reacted with amyloid in all regions in the serial sections in which Congo red staining was positive. A highly sensitive 27-MHz quartz crystal microbalance analysis revealed that BSB showed a significant affinity for amyloid fibrils purified from familial amyloidotic polyneuropathy and dialysis-related amyloidosis samples and suppressed formation of transthyretin amyloid in vitro. These results suggest that BSB may become a valuable tool for detection of amyloid deposits in amyloidosis and of the mechanism of amyloid formation.
AB - We synthesized (trans, trans)-1-bromo-2,5-bis-(3-hydroxycarbonyl-4-hydroxy)styrylbenzene (BSB) and used this compound to detect amyloid fibrils in autopsy and biopsy samples from patients with localized amyloidosis, such as familial prion disease, and systemic amyloidosis, such as familial amyloidotic polyneuropathy, amyloid A (AA) amyloidosis, light chain (AL) amyloidosis, and dialysis-related amyloidosis. BSB showed reactions in all Congo red-positive and immunoreactive regions of the samples examined in the study, and some amyloid fibrils in the tissues could be detected more precisely with BSB than with the other methods. In the mouse model of AA amyloidosis, injected BSB reacted with amyloid in all regions in the serial sections in which Congo red staining was positive. A highly sensitive 27-MHz quartz crystal microbalance analysis revealed that BSB showed a significant affinity for amyloid fibrils purified from familial amyloidotic polyneuropathy and dialysis-related amyloidosis samples and suppressed formation of transthyretin amyloid in vitro. These results suggest that BSB may become a valuable tool for detection of amyloid deposits in amyloidosis and of the mechanism of amyloid formation.
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U2 - 10.1097/01.LAB.0000101701.87433.C5
DO - 10.1097/01.LAB.0000101701.87433.C5
M3 - Article
C2 - 14691293
AN - SCOPUS:9144262344
SN - 0023-6837
VL - 83
SP - 1751
EP - 1759
JO - Laboratory Investigation
JF - Laboratory Investigation
IS - 12
ER -