TY - JOUR
T1 - A preadipocyte clonal line from bovine intramuscular adipose tissue
T2 - Nonexpression of glut-4 protein during adipocyte differentiation
AU - Aso, Hisashi
AU - Abe, Hiroyuki
AU - Nakajima, Ikuyo
AU - Ozutsumi, Kyouhei
AU - Yamaguchi, Takahiro
AU - Takamori, Yasushi
AU - Kodama, Akira
AU - Hoshino, Fuminori B.
AU - Takano, Shoichi
PY - 1995
Y1 - 1995
N2 - A clonal bovine intramuscular preadipocyte (BIP) line has been established from the intramuscular white adipose tissue of the M. longissimus thoracis in each of three Japanese Black cattle. Exponentially growing BIP cells exhibited a fibroblastic appearance. Adipocyte differentiation was initiated by treating confluent BIP cells with differentiation medium containing insulin and dexamethasone. Small lipid droplets appeared 5-6 days after stimulation and occupied a large fraction of the cell volume at 10 days and beyond. During the adipose conversion, the incorporation of acetate to the cells gradually increased by 10-fold and reached a maximum at day 5. However, incorporation of glucose increased only S-folds prior to this conversion, even though GLUT-1 level increased by 13-fold at day 7. GLUT-4 on the other hand, was not detected during the course of differentiation. These results suggested that adipose tissue metabolisms in ruminants were different from that of non-ruminants.
AB - A clonal bovine intramuscular preadipocyte (BIP) line has been established from the intramuscular white adipose tissue of the M. longissimus thoracis in each of three Japanese Black cattle. Exponentially growing BIP cells exhibited a fibroblastic appearance. Adipocyte differentiation was initiated by treating confluent BIP cells with differentiation medium containing insulin and dexamethasone. Small lipid droplets appeared 5-6 days after stimulation and occupied a large fraction of the cell volume at 10 days and beyond. During the adipose conversion, the incorporation of acetate to the cells gradually increased by 10-fold and reached a maximum at day 5. However, incorporation of glucose increased only S-folds prior to this conversion, even though GLUT-1 level increased by 13-fold at day 7. GLUT-4 on the other hand, was not detected during the course of differentiation. These results suggested that adipose tissue metabolisms in ruminants were different from that of non-ruminants.
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U2 - 10.1006/bbrc.1995.2141
DO - 10.1006/bbrc.1995.2141
M3 - Article
C2 - 7646489
AN - SCOPUS:0029128608
SN - 0006-291X
VL - 213
SP - 369
EP - 375
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -