A Rat Model for Monitoring Homograft Rejection of the Lung

Takashi Kondo; Fumihiro Shionozaki; Shigefumi Fujimura; Atsushi Yamauchi; Masashi Handa; Takeshi Okabe; Ryo Saito; Takashi Ichinose; Yuji Shiraishi; Tasuku Nakada

doi:10.1620/tjem.149.323

A Rat Model for Monitoring Homograft Rejection of the Lung

Takashi Kondo, Fumihiro Shionozaki, Shigefumi Fujimura, Atsushi Yamauchi, Masashi Handa, Takeshi Okabe, Ryo Saito, Takashi Ichinose, Yuji Shiraishi, Tasuku Nakada

Institute of Development, Aging and Cancer (IDAC)

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

A new surgical procedure was devised for the experimental homograft of the lung. The pulmonary artery and vein and bronchus were anastomosed under the operation microscope using interrupted sutures in group W (30 outbred Wistar rats) and continuous sutures in group F (7 inbred F344/Ducrj Rats). Mean ischemia time during the operation was 95.3±10.3 min in the group W and 55.6 ± 5.8 in group F. Eight out of 30 rats (27%) of group W and all rats of group F survived more than 24 hr after the surgery. Pulmonary functions of grafted lungs were evaluated by serial x-rays and the perfusion ratio was 27.4±11.6% (mean± S.D.) in group W and 25.4± 7.7% in group F. A patent opening of the hilar anastomosis in these rats was confirmed at autopsy. This model with continuous suture would be useful for the rat lung transplantation.

Original language	English
Pages (from-to)	323-328
Number of pages	6
Journal	the tohoku journal of experimental medicine
Volume	149
Issue number	3
DOIs	https://doi.org/10.1620/tjem.149.323
Publication status	Published - 1986

Keywords

microsurgery
rat lung transplantation

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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10.1620/tjem.149.323

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title = "A Rat Model for Monitoring Homograft Rejection of the Lung",

abstract = "A new surgical procedure was devised for the experimental homograft of the lung. The pulmonary artery and vein and bronchus were anastomosed under the operation microscope using interrupted sutures in group W (30 outbred Wistar rats) and continuous sutures in group F (7 inbred F344/Ducrj Rats). Mean ischemia time during the operation was 95.3±10.3 min in the group W and 55.6 ± 5.8 in group F. Eight out of 30 rats (27%) of group W and all rats of group F survived more than 24 hr after the surgery. Pulmonary functions of grafted lungs were evaluated by serial x-rays and the perfusion ratio was 27.4±11.6% (mean± S.D.) in group W and 25.4± 7.7% in group F. A patent opening of the hilar anastomosis in these rats was confirmed at autopsy. This model with continuous suture would be useful for the rat lung transplantation.",

keywords = "microsurgery, rat lung transplantation",

author = "Takashi Kondo and Fumihiro Shionozaki and Shigefumi Fujimura and Atsushi Yamauchi and Masashi Handa and Takeshi Okabe and Ryo Saito and Takashi Ichinose and Yuji Shiraishi and Tasuku Nakada",

year = "1986",

doi = "10.1620/tjem.149.323",

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journal = "Tohoku Journal of Experimental Medicine",

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T1 - A Rat Model for Monitoring Homograft Rejection of the Lung

AU - Kondo, Takashi

AU - Shionozaki, Fumihiro

AU - Fujimura, Shigefumi

AU - Yamauchi, Atsushi

AU - Handa, Masashi

AU - Okabe, Takeshi

AU - Saito, Ryo

AU - Ichinose, Takashi

AU - Shiraishi, Yuji

AU - Nakada, Tasuku

PY - 1986

Y1 - 1986

N2 - A new surgical procedure was devised for the experimental homograft of the lung. The pulmonary artery and vein and bronchus were anastomosed under the operation microscope using interrupted sutures in group W (30 outbred Wistar rats) and continuous sutures in group F (7 inbred F344/Ducrj Rats). Mean ischemia time during the operation was 95.3±10.3 min in the group W and 55.6 ± 5.8 in group F. Eight out of 30 rats (27%) of group W and all rats of group F survived more than 24 hr after the surgery. Pulmonary functions of grafted lungs were evaluated by serial x-rays and the perfusion ratio was 27.4±11.6% (mean± S.D.) in group W and 25.4± 7.7% in group F. A patent opening of the hilar anastomosis in these rats was confirmed at autopsy. This model with continuous suture would be useful for the rat lung transplantation.

AB - A new surgical procedure was devised for the experimental homograft of the lung. The pulmonary artery and vein and bronchus were anastomosed under the operation microscope using interrupted sutures in group W (30 outbred Wistar rats) and continuous sutures in group F (7 inbred F344/Ducrj Rats). Mean ischemia time during the operation was 95.3±10.3 min in the group W and 55.6 ± 5.8 in group F. Eight out of 30 rats (27%) of group W and all rats of group F survived more than 24 hr after the surgery. Pulmonary functions of grafted lungs were evaluated by serial x-rays and the perfusion ratio was 27.4±11.6% (mean± S.D.) in group W and 25.4± 7.7% in group F. A patent opening of the hilar anastomosis in these rats was confirmed at autopsy. This model with continuous suture would be useful for the rat lung transplantation.

KW - microsurgery

KW - rat lung transplantation

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JO - Tohoku Journal of Experimental Medicine

JF - Tohoku Journal of Experimental Medicine

IS - 3

ER -

A Rat Model for Monitoring Homograft Rejection of the Lung

Abstract

Keywords

ASJC Scopus subject areas

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