TY - JOUR
T1 - A rationally designed JAZ subtype-selective agonist of jasmonate perception
AU - Takaoka, Yousuke
AU - Iwahashi, Mana
AU - Chini, Andrea
AU - Saito, Hiroaki
AU - Ishimaru, Yasuhiro
AU - Egoshi, Syusuke
AU - Kato, Nobuki
AU - Tanaka, Maho
AU - Bashir, Khurram
AU - Seki, Motoaki
AU - Solano, Roberto
AU - Ueda, Minoru
N1 - Funding Information:
We thank Prof. E. Kombrink (Max Planck Institute for Plant Breeding Research, Köln) for providing us with seeds for the jar1 mutant, and Mr. K. Hayashi, Mr. T. Iwashita, and Mr. K. Julien (Tohoku Univ.) for technical assistance. The seed of jazQ was kindly provided by Prof. Gregg Howe (Michigan State Univ.). This work was supported by a Grant-in-Aid for Scientific Research on Innovative Areas (no. 23102012) ‘Chemical Biology of Natural Products (no. 2301)’ for M.U. from MEXT, Japan, a Grant-in-Aid for Scientific Research (no. 26282207, 17H06407, and no. 17H00885 to M.U.), JSPS A3 Foresight Program (to M.U.), Naito Foundation (to M.U.), JST (JPMJPR16Q4 to Y.T.), JST CREST (JPMJCR13B4 to M.S.), and Spanish Ministry for Science and Innovation grant BIO2016-77216-R (MINECO/FEDER) for A.C. and R.S. This study was supported by JSPS KAKENHI (JP16K05648 to H.S.). The computations in this study were performed using the advanced center for computing and communication of RIKEN, the research center for computational science of Institute for Molecular Science (IMS), the research center for advanced computing infrastructure of Japan Advanced Institute of Science and Technology (JAIST), and the center for computational science of Tsukuba university.
Publisher Copyright:
© 2018, The Author(s).
PY - 2018/12/1
Y1 - 2018/12/1
N2 - The phytohormone 7-iso-(+)-jasmonoyl-L-isoleucine (JA-Ile) mediates plant defense responses against herbivore and pathogen attack, and thus increases plant resistance against foreign invaders. However, JA-Ile also causes growth inhibition; and therefore JA-Ile is not a practical chemical regulator of plant defense responses. Here, we describe the rational design and synthesis of a small molecule agonist that can upregulate defense-related gene expression and promote pathogen resistance at concentrations that do not cause growth inhibition in Arabidopsis. By stabilizing interactions between COI1 and JAZ9 and JAZ10 but no other JAZ isoforms, the agonist leads to formation of JA-Ile co-receptors that selectively activate the JAZ9-EIN3/EIL1-ORA59 signaling pathway. The design of a JA-Ile agonist with high selectivity for specific protein subtypes may help promote the development of chemical regulators that do not cause a tradeoff between growth and defense.
AB - The phytohormone 7-iso-(+)-jasmonoyl-L-isoleucine (JA-Ile) mediates plant defense responses against herbivore and pathogen attack, and thus increases plant resistance against foreign invaders. However, JA-Ile also causes growth inhibition; and therefore JA-Ile is not a practical chemical regulator of plant defense responses. Here, we describe the rational design and synthesis of a small molecule agonist that can upregulate defense-related gene expression and promote pathogen resistance at concentrations that do not cause growth inhibition in Arabidopsis. By stabilizing interactions between COI1 and JAZ9 and JAZ10 but no other JAZ isoforms, the agonist leads to formation of JA-Ile co-receptors that selectively activate the JAZ9-EIN3/EIL1-ORA59 signaling pathway. The design of a JA-Ile agonist with high selectivity for specific protein subtypes may help promote the development of chemical regulators that do not cause a tradeoff between growth and defense.
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U2 - 10.1038/s41467-018-06135-y
DO - 10.1038/s41467-018-06135-y
M3 - Article
C2 - 30194307
AN - SCOPUS:85053007702
SN - 2041-1723
VL - 9
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 3654
ER -