A sequence-ready contig map of the top arm of Arabidopsis thaliana chromosome 3

Shusei Sato; Takakazu Kaneko; Hirokazu Kotani; Reiko Hayashi; Yao Guang Liu; Daisuke Shibata; Satoshi Tabata

doi:10.1093/dnares/6.2.117

A sequence-ready contig map of the top arm of Arabidopsis thaliana chromosome 3

Shusei Sato, Takakazu Kaneko, Hirokazu Kotani, Reiko Hayashi, Yao Guang Liu, Daisuke Shibata, Satoshi Tabata

LIF - Ecological Developmental Adaptability Life Sciences

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

A fine physical map of the top arm of Arabidopsis thaliana chromosome 3 has been constructed by ordering PI, TAC and BAC clones using the sequences of a variety of DNA markers and end-sequences of clones. The marker sequences used in this study were derived from 58 DNA markers, 93 YAC end-sequences, and 807 end-sequences of P1, TAC and BAC clones. The entire top arm of chromosome 3, except for the centromeric and telomeric regions, was covered by a single contig 13.3 Mb long. This fine physical map will facilitate gene isolation by map-based cloning experiments as well as genome sequencing of the top arm of chromosome 3. The map and end-sequence information are available on the web site KAOS (Kazusa Arabidopsis data Opening Site) at [http://www.kazusa.or.jp/arabi/].

Original language	English
Pages (from-to)	117-121
Number of pages	5
Journal	DNA Research
Volume	6
Issue number	2
DOIs	https://doi.org/10.1093/dnares/6.2.117
Publication status	Published - 1999

Keywords

Arabidopsis thaliana
Chromosome 3
Physical map
Sequence-ready contig map

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10.1093/dnares/6.2.117

Cite this

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abstract = "A fine physical map of the top arm of Arabidopsis thaliana chromosome 3 has been constructed by ordering PI, TAC and BAC clones using the sequences of a variety of DNA markers and end-sequences of clones. The marker sequences used in this study were derived from 58 DNA markers, 93 YAC end-sequences, and 807 end-sequences of P1, TAC and BAC clones. The entire top arm of chromosome 3, except for the centromeric and telomeric regions, was covered by a single contig 13.3 Mb long. This fine physical map will facilitate gene isolation by map-based cloning experiments as well as genome sequencing of the top arm of chromosome 3. The map and end-sequence information are available on the web site KAOS (Kazusa Arabidopsis data Opening Site) at [http://www.kazusa.or.jp/arabi/].",

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T1 - A sequence-ready contig map of the top arm of Arabidopsis thaliana chromosome 3

AU - Sato, Shusei

AU - Kaneko, Takakazu

AU - Kotani, Hirokazu

AU - Hayashi, Reiko

AU - Liu, Yao Guang

AU - Shibata, Daisuke

AU - Tabata, Satoshi

PY - 1999

Y1 - 1999

N2 - A fine physical map of the top arm of Arabidopsis thaliana chromosome 3 has been constructed by ordering PI, TAC and BAC clones using the sequences of a variety of DNA markers and end-sequences of clones. The marker sequences used in this study were derived from 58 DNA markers, 93 YAC end-sequences, and 807 end-sequences of P1, TAC and BAC clones. The entire top arm of chromosome 3, except for the centromeric and telomeric regions, was covered by a single contig 13.3 Mb long. This fine physical map will facilitate gene isolation by map-based cloning experiments as well as genome sequencing of the top arm of chromosome 3. The map and end-sequence information are available on the web site KAOS (Kazusa Arabidopsis data Opening Site) at [http://www.kazusa.or.jp/arabi/].

AB - A fine physical map of the top arm of Arabidopsis thaliana chromosome 3 has been constructed by ordering PI, TAC and BAC clones using the sequences of a variety of DNA markers and end-sequences of clones. The marker sequences used in this study were derived from 58 DNA markers, 93 YAC end-sequences, and 807 end-sequences of P1, TAC and BAC clones. The entire top arm of chromosome 3, except for the centromeric and telomeric regions, was covered by a single contig 13.3 Mb long. This fine physical map will facilitate gene isolation by map-based cloning experiments as well as genome sequencing of the top arm of chromosome 3. The map and end-sequence information are available on the web site KAOS (Kazusa Arabidopsis data Opening Site) at [http://www.kazusa.or.jp/arabi/].

KW - Arabidopsis thaliana

KW - Chromosome 3

KW - Physical map

KW - Sequence-ready contig map

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A sequence-ready contig map of the top arm of Arabidopsis thaliana chromosome 3

Abstract

Keywords

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