A simple and rapid detection system for oral bacteria in liquid phase for point-of-care diagnostics using magnetic nanoparticles

Loi Tonthat; Shunnosuke Takahashi; Hidehiko Onodera; Kazuhiko Okita; Shin Yabukami; Kotone Yokota; Maiko Furuya; Hiroyasu Kanetaka; Yoshinori Miura; Hideki Takahashi; Yoshihiko Watanabe; Ritsuko Akiyama

doi:10.1063/1.5130437

A simple and rapid detection system for oral bacteria in liquid phase for point-of-care diagnostics using magnetic nanoparticles

Loi Tonthat, Shunnosuke Takahashi, Hidehiko Onodera, Kazuhiko Okita, Shin Yabukami, Kotone Yokota, Maiko Furuya, Hiroyasu Kanetaka, Yoshinori Miura, Hideki Takahashi, Yoshihiko Watanabe, Ritsuko Akiyama

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

This study describes a user-friendly and rapid detection system of oral bacteria in the liquid phase for point of care testing based on magnetic immunoassay. We focused on the dependence of the strength of external magnetic field required to switch the magnetic moments of nanoparticles bound to bacteria on the bacteria concentration. The results obtained indicate that the required field strength increases linearly as a function of log concentration of Porphyromonas gingivalis cultured in the range of 10³-10⁹ CFU/mL. Similarly, the required field strengths for Streptococcus mutans and Pseudomonas aeruginosa increase monotonically when their concentrations increase, whereas the required field strength for Escherichia coli decreases monotonically when its concentration increases. We then measured the concentration of Porphyromonas gingivalis in the saliva collected from elderly people in a geriatric health services facility and the results using the developed system had a correlation with those using a commercial bacteria counter.

Original language	English
Article number	125325
Journal	AIP Advances
Volume	9
Issue number	12
DOIs	https://doi.org/10.1063/1.5130437
Publication status	Published - 2019 Dec 1

Access to Document

10.1063/1.5130437

Cite this

@article{7a6583a6e6b642bda2753df20082ad3e,

title = "A simple and rapid detection system for oral bacteria in liquid phase for point-of-care diagnostics using magnetic nanoparticles",

abstract = "This study describes a user-friendly and rapid detection system of oral bacteria in the liquid phase for point of care testing based on magnetic immunoassay. We focused on the dependence of the strength of external magnetic field required to switch the magnetic moments of nanoparticles bound to bacteria on the bacteria concentration. The results obtained indicate that the required field strength increases linearly as a function of log concentration of Porphyromonas gingivalis cultured in the range of 103-109 CFU/mL. Similarly, the required field strengths for Streptococcus mutans and Pseudomonas aeruginosa increase monotonically when their concentrations increase, whereas the required field strength for Escherichia coli decreases monotonically when its concentration increases. We then measured the concentration of Porphyromonas gingivalis in the saliva collected from elderly people in a geriatric health services facility and the results using the developed system had a correlation with those using a commercial bacteria counter.",

author = "Loi Tonthat and Shunnosuke Takahashi and Hidehiko Onodera and Kazuhiko Okita and Shin Yabukami and Kotone Yokota and Maiko Furuya and Hiroyasu Kanetaka and Yoshinori Miura and Hideki Takahashi and Yoshihiko Watanabe and Ritsuko Akiyama",

note = "Funding Information: This work was supported in part by the Center of Innovation Science and Technology based Radical Innovation and Entrepreneurship Program (COI STREAM), and Adaptable and Seamless Technology Transfer Program (A-STEP) from Japan Science and Technology Agency (JST) and the Frontier Research Institute for Interdisciplinary Sciences of Tohoku University. The authors would also like to thank Prof. Nobuhiro Takahashi, and Dr. Jumpei Washio of the Division of Oral Ecology and Biochemistry, Graduate School of Dentistry, Tohoku University for their assistance in bacterial culture. Publisher Copyright: {\textcopyright} 2019 Author(s).",

year = "2019",

month = dec,

day = "1",

doi = "10.1063/1.5130437",

language = "English",

volume = "9",

journal = "AIP Advances",

issn = "2158-3226",

publisher = "American Institute of Physics",

number = "12",

}

TY - JOUR

T1 - A simple and rapid detection system for oral bacteria in liquid phase for point-of-care diagnostics using magnetic nanoparticles

AU - Tonthat, Loi

AU - Takahashi, Shunnosuke

AU - Onodera, Hidehiko

AU - Okita, Kazuhiko

AU - Yabukami, Shin

AU - Yokota, Kotone

AU - Furuya, Maiko

AU - Kanetaka, Hiroyasu

AU - Miura, Yoshinori

AU - Takahashi, Hideki

AU - Watanabe, Yoshihiko

AU - Akiyama, Ritsuko

N1 - Funding Information: This work was supported in part by the Center of Innovation Science and Technology based Radical Innovation and Entrepreneurship Program (COI STREAM), and Adaptable and Seamless Technology Transfer Program (A-STEP) from Japan Science and Technology Agency (JST) and the Frontier Research Institute for Interdisciplinary Sciences of Tohoku University. The authors would also like to thank Prof. Nobuhiro Takahashi, and Dr. Jumpei Washio of the Division of Oral Ecology and Biochemistry, Graduate School of Dentistry, Tohoku University for their assistance in bacterial culture. Publisher Copyright: © 2019 Author(s).

PY - 2019/12/1

Y1 - 2019/12/1

N2 - This study describes a user-friendly and rapid detection system of oral bacteria in the liquid phase for point of care testing based on magnetic immunoassay. We focused on the dependence of the strength of external magnetic field required to switch the magnetic moments of nanoparticles bound to bacteria on the bacteria concentration. The results obtained indicate that the required field strength increases linearly as a function of log concentration of Porphyromonas gingivalis cultured in the range of 103-109 CFU/mL. Similarly, the required field strengths for Streptococcus mutans and Pseudomonas aeruginosa increase monotonically when their concentrations increase, whereas the required field strength for Escherichia coli decreases monotonically when its concentration increases. We then measured the concentration of Porphyromonas gingivalis in the saliva collected from elderly people in a geriatric health services facility and the results using the developed system had a correlation with those using a commercial bacteria counter.

AB - This study describes a user-friendly and rapid detection system of oral bacteria in the liquid phase for point of care testing based on magnetic immunoassay. We focused on the dependence of the strength of external magnetic field required to switch the magnetic moments of nanoparticles bound to bacteria on the bacteria concentration. The results obtained indicate that the required field strength increases linearly as a function of log concentration of Porphyromonas gingivalis cultured in the range of 103-109 CFU/mL. Similarly, the required field strengths for Streptococcus mutans and Pseudomonas aeruginosa increase monotonically when their concentrations increase, whereas the required field strength for Escherichia coli decreases monotonically when its concentration increases. We then measured the concentration of Porphyromonas gingivalis in the saliva collected from elderly people in a geriatric health services facility and the results using the developed system had a correlation with those using a commercial bacteria counter.

UR - http://www.scopus.com/inward/record.url?scp=85077316657&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85077316657&partnerID=8YFLogxK

U2 - 10.1063/1.5130437

DO - 10.1063/1.5130437

M3 - Article

AN - SCOPUS:85077316657

SN - 2158-3226

VL - 9

JO - AIP Advances

JF - AIP Advances

IS - 12

M1 - 125325

ER -

A simple and rapid detection system for oral bacteria in liquid phase for point-of-care diagnostics using magnetic nanoparticles

Abstract

Access to Document

Other files and links

Fingerprint

Cite this