A single nucleotide polymorphism in the alternative oxidase gene among rice varieties differing in low temperature tolerance

Fumitaka Abe; Koji Saito; Kiyoyuki Miura; Kinya Toriyama

doi:10.1016/S0014-5793(02)03218-0

A single nucleotide polymorphism in the alternative oxidase gene among rice varieties differing in low temperature tolerance

Fumitaka Abe, Koji Saito, Kiyoyuki Miura, Kinya Toriyama

AGR - Agricultural Bioscience

Research output: Contribution to journal › Article › peer-review

45 Citations (Scopus)

Abstract

Alternative oxidase (AOX) is encoded in a multigene family, and multiple isoforms have been observed in various plant species. We found for the first time an allelic variation in the same AOX locus. On SDS-gel blots of callus protein of rice (Oryza sativa L.), varieties without the QTL for low temperature tolerance showed a 32-kDa AOX band, whereas those with the QTL showed a 34-kDa band. The variation was attributed to the substitution of Lys⁷¹ for Asn⁷¹ caused by a single nucleotide polymorphism between alleles of OsAOX1a, and was tightly linked to the presence of the QTL.

Original language	English
Pages (from-to)	181-185
Number of pages	5
Journal	FEBS Letters
Volume	527
Issue number	1-3
DOIs	https://doi.org/10.1016/S0014-5793(02)03218-0
Publication status	Published - 2002 Sept 11

Keywords

Alternative oxidase
Isoforms
Low temperature tolerance
Oryza sativa L
Single nucleotide polymorphism

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10.1016/S0014-5793(02)03218-0

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title = "A single nucleotide polymorphism in the alternative oxidase gene among rice varieties differing in low temperature tolerance",

abstract = "Alternative oxidase (AOX) is encoded in a multigene family, and multiple isoforms have been observed in various plant species. We found for the first time an allelic variation in the same AOX locus. On SDS-gel blots of callus protein of rice (Oryza sativa L.), varieties without the QTL for low temperature tolerance showed a 32-kDa AOX band, whereas those with the QTL showed a 34-kDa band. The variation was attributed to the substitution of Lys71 for Asn71 caused by a single nucleotide polymorphism between alleles of OsAOX1a, and was tightly linked to the presence of the QTL.",

keywords = "Alternative oxidase, Isoforms, Low temperature tolerance, Oryza sativa L, Single nucleotide polymorphism",

author = "Fumitaka Abe and Koji Saito and Kiyoyuki Miura and Kinya Toriyama",

note = "Funding Information: The authors thank Dr. Cecil R. Stewart for the gift of the antibodies provided by Dr. Thomas E. Elthon, and Dr. Masahiro Yano, Rice Genome Research program, National Institute of Agrobiological Resources, Japan for the mapping of the OsAOX1a . This work was supported in part by a grant for Recombinant Plant Project from the Ministry of Agriculture, Forestry and Fisheries, Japan. ",

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doi = "10.1016/S0014-5793(02)03218-0",

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journal = "FEBS Letters",

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T1 - A single nucleotide polymorphism in the alternative oxidase gene among rice varieties differing in low temperature tolerance

AU - Abe, Fumitaka

AU - Saito, Koji

AU - Miura, Kiyoyuki

AU - Toriyama, Kinya

N1 - Funding Information: The authors thank Dr. Cecil R. Stewart for the gift of the antibodies provided by Dr. Thomas E. Elthon, and Dr. Masahiro Yano, Rice Genome Research program, National Institute of Agrobiological Resources, Japan for the mapping of the OsAOX1a . This work was supported in part by a grant for Recombinant Plant Project from the Ministry of Agriculture, Forestry and Fisheries, Japan.

PY - 2002/9/11

Y1 - 2002/9/11

N2 - Alternative oxidase (AOX) is encoded in a multigene family, and multiple isoforms have been observed in various plant species. We found for the first time an allelic variation in the same AOX locus. On SDS-gel blots of callus protein of rice (Oryza sativa L.), varieties without the QTL for low temperature tolerance showed a 32-kDa AOX band, whereas those with the QTL showed a 34-kDa band. The variation was attributed to the substitution of Lys71 for Asn71 caused by a single nucleotide polymorphism between alleles of OsAOX1a, and was tightly linked to the presence of the QTL.

AB - Alternative oxidase (AOX) is encoded in a multigene family, and multiple isoforms have been observed in various plant species. We found for the first time an allelic variation in the same AOX locus. On SDS-gel blots of callus protein of rice (Oryza sativa L.), varieties without the QTL for low temperature tolerance showed a 32-kDa AOX band, whereas those with the QTL showed a 34-kDa band. The variation was attributed to the substitution of Lys71 for Asn71 caused by a single nucleotide polymorphism between alleles of OsAOX1a, and was tightly linked to the presence of the QTL.

KW - Alternative oxidase

KW - Isoforms

KW - Low temperature tolerance

KW - Oryza sativa L

KW - Single nucleotide polymorphism

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DO - 10.1016/S0014-5793(02)03218-0

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EP - 185

JO - FEBS Letters

JF - FEBS Letters

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ER -

A single nucleotide polymorphism in the alternative oxidase gene among rice varieties differing in low temperature tolerance

Abstract

Keywords

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