TY - JOUR
T1 - A soluble nonglycosylated recombinant infectious hematopoietic necrosis virus (IHNV) G-protein induces IFNs in rainbow trout (Oncorhynchus mykiss)
AU - Verjan, Noel
AU - Ooi, Ei Lin
AU - Nochi, Tomonori
AU - Kondo, Hidehiro
AU - Hirono, Ikuo
AU - Aoki, Takashi
AU - Kiyono, Hiroshi
AU - Yuki, Yoshikazu
N1 - Funding Information:
This work was supported by grants from the Japan Science and Technology Corporation's Creation and Support Program for Start-ups from Universities.
PY - 2008/7
Y1 - 2008/7
N2 - Viral glycoproteins interact with cell-surface receptors to mediate virus entry and innate immune system activation. We found that a soluble recombinant infectious hematopoietic necrosis virus G-protein (rIHNV-G) stimulated an early innate immune response mediated by proinflammatory cytokines, IFN1 and IFN-γ in rainbow trout (Oncorhynchus mykiss) fry. Expression of both IFN1 and IFN-γ mRNA transcripts was an early event and was rIHNV-G dose-dependent. In addition, preliminary evidence revealed that the innate immune response induced by rIHNV-G protein could protect rainbow trout fry from a subsequent IHNV virus challenge. Finally, the binding and distribution of FITC-rIHNV-G protein on rainbow trout spleen and head kidney leukocytes resemble morphological changes which occur on the cell membrane during antigen-receptor interaction including membrane reorganization, patching, polarization and capping. Thus a soluble nonglycosylated rIHNV-G protein could mediate the activation of rainbow trout leukocytes, with concomitant production of proinflammatory cytokines and IFNs.
AB - Viral glycoproteins interact with cell-surface receptors to mediate virus entry and innate immune system activation. We found that a soluble recombinant infectious hematopoietic necrosis virus G-protein (rIHNV-G) stimulated an early innate immune response mediated by proinflammatory cytokines, IFN1 and IFN-γ in rainbow trout (Oncorhynchus mykiss) fry. Expression of both IFN1 and IFN-γ mRNA transcripts was an early event and was rIHNV-G dose-dependent. In addition, preliminary evidence revealed that the innate immune response induced by rIHNV-G protein could protect rainbow trout fry from a subsequent IHNV virus challenge. Finally, the binding and distribution of FITC-rIHNV-G protein on rainbow trout spleen and head kidney leukocytes resemble morphological changes which occur on the cell membrane during antigen-receptor interaction including membrane reorganization, patching, polarization and capping. Thus a soluble nonglycosylated rIHNV-G protein could mediate the activation of rainbow trout leukocytes, with concomitant production of proinflammatory cytokines and IFNs.
KW - IFN
KW - IHNV
KW - Innate immune response
KW - Leukocyte activation
KW - Recombinant G protein
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U2 - 10.1016/j.fsi.2008.04.004
DO - 10.1016/j.fsi.2008.04.004
M3 - Article
C2 - 18499475
AN - SCOPUS:44749092713
SN - 1050-4648
VL - 25
SP - 170
EP - 180
JO - Fish and Shellfish Immunology
JF - Fish and Shellfish Immunology
IS - 1-2
ER -