ABCA3-mediated choline-phospholipids uptake into intracellular vesicles in A549 cells

Yoshihiro Matsumura, Hiromichi Sakai, Mayumi Sasaki, Nobuhiro Ban, Nobuya Inagaki

Research output: Contribution to journalArticlepeer-review

55 Citations (Scopus)


ABCA3 is proposed to function as a lung surfactant lipid transporter. Here we report ABCA3-dependent lipid uptake into intracellular vesicles in lung adenocarcinoma A549 cells. A549 cells stably expressing GFP-tagged wild-type ABCA3 (A549/ABCA3WT) had larger LAMP3-positive vesicles than their parental cells as well as A549 cells expressing a Walker A motif mutant (A549/ABCA3N568D). The choline-phospholipids level in A549/ABCA3WT was increased 1.25-fold compared to that in A549 and A549/ABCA3N568D cells, while the cholesterol levels were similar. Sucrose gradient fractionation analysis in A549/ABCA3WT cells revealed that choline-phospholipids were enriched in low-density and nile red-positive vesicles. Electronmicroscopic analysis showed multilamellar vesicles in A549/ABCA3WT cells. These results indicate that ABCA3 mediates ATP-dependent choline-phospholipids uptake into intracellular vesicles.

Original languageEnglish
Pages (from-to)3139-3144
Number of pages6
JournalFEBS Letters
Issue number17
Publication statusPublished - 2007 Jul 10
Externally publishedYes


  • Alveolar type II cell
  • ATP-binding cassette transporter
  • Choline-phospholipids
  • Lamellar body
  • Lipid transport
  • Surfactant

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology


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