ABCA3-mediated choline-phospholipids uptake into intracellular vesicles in A549 cells

Yoshihiro Matsumura; Hiromichi Sakai; Mayumi Sasaki; Nobuhiro Ban; Nobuya Inagaki

doi:10.1016/j.febslet.2007.05.078

ABCA3-mediated choline-phospholipids uptake into intracellular vesicles in A549 cells

Yoshihiro Matsumura, Hiromichi Sakai, Mayumi Sasaki, Nobuhiro Ban, Nobuya Inagaki

Research output: Contribution to journal › Article › peer-review

55 Citations (Scopus)

Abstract

ABCA3 is proposed to function as a lung surfactant lipid transporter. Here we report ABCA3-dependent lipid uptake into intracellular vesicles in lung adenocarcinoma A549 cells. A549 cells stably expressing GFP-tagged wild-type ABCA3 (A549/ABCA3^WT) had larger LAMP3-positive vesicles than their parental cells as well as A549 cells expressing a Walker A motif mutant (A549/ABCA3^N568D). The choline-phospholipids level in A549/ABCA3^WT was increased 1.25-fold compared to that in A549 and A549/ABCA3^N568D cells, while the cholesterol levels were similar. Sucrose gradient fractionation analysis in A549/ABCA3^WT cells revealed that choline-phospholipids were enriched in low-density and nile red-positive vesicles. Electronmicroscopic analysis showed multilamellar vesicles in A549/ABCA3^WT cells. These results indicate that ABCA3 mediates ATP-dependent choline-phospholipids uptake into intracellular vesicles.

Original language	English
Pages (from-to)	3139-3144
Number of pages	6
Journal	FEBS Letters
Volume	581
Issue number	17
DOIs	https://doi.org/10.1016/j.febslet.2007.05.078
Publication status	Published - 2007 Jul 10
Externally published	Yes

Keywords

Alveolar type II cell
ATP-binding cassette transporter
Choline-phospholipids
Lamellar body
Lipid transport
Surfactant

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2007.05.078

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title = "ABCA3-mediated choline-phospholipids uptake into intracellular vesicles in A549 cells",

abstract = "ABCA3 is proposed to function as a lung surfactant lipid transporter. Here we report ABCA3-dependent lipid uptake into intracellular vesicles in lung adenocarcinoma A549 cells. A549 cells stably expressing GFP-tagged wild-type ABCA3 (A549/ABCA3WT) had larger LAMP3-positive vesicles than their parental cells as well as A549 cells expressing a Walker A motif mutant (A549/ABCA3N568D). The choline-phospholipids level in A549/ABCA3WT was increased 1.25-fold compared to that in A549 and A549/ABCA3N568D cells, while the cholesterol levels were similar. Sucrose gradient fractionation analysis in A549/ABCA3WT cells revealed that choline-phospholipids were enriched in low-density and nile red-positive vesicles. Electronmicroscopic analysis showed multilamellar vesicles in A549/ABCA3WT cells. These results indicate that ABCA3 mediates ATP-dependent choline-phospholipids uptake into intracellular vesicles.",

keywords = "Alveolar type II cell, ATP-binding cassette transporter, Choline-phospholipids, Lamellar body, Lipid transport, Surfactant",

author = "Yoshihiro Matsumura and Hiromichi Sakai and Mayumi Sasaki and Nobuhiro Ban and Nobuya Inagaki",

note = "Funding Information: This work was supported by Scientific Research Grants and a Grant-in-Aid for Creative Scientific Research (15GS0301) from the Ministry of Education, Culture. Sports, Science and Technology of Japan; CREST (Core Research for Evolutional Science and Technology) of Japan Science and Technology Agency; and the 21st Century Center of Excellence Program. We thank Dr. Ichiro Yoshida for help in the initial stage of the work, Dr. Yoshio Kuroki for anti-mature SP-B antibody, and Mr. Shinsuke Chida for preparation of electronmicroscopy samples. ",

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AU - Matsumura, Yoshihiro

AU - Sakai, Hiromichi

AU - Sasaki, Mayumi

AU - Ban, Nobuhiro

AU - Inagaki, Nobuya

N1 - Funding Information: This work was supported by Scientific Research Grants and a Grant-in-Aid for Creative Scientific Research (15GS0301) from the Ministry of Education, Culture. Sports, Science and Technology of Japan; CREST (Core Research for Evolutional Science and Technology) of Japan Science and Technology Agency; and the 21st Century Center of Excellence Program. We thank Dr. Ichiro Yoshida for help in the initial stage of the work, Dr. Yoshio Kuroki for anti-mature SP-B antibody, and Mr. Shinsuke Chida for preparation of electronmicroscopy samples.

PY - 2007/7/10

Y1 - 2007/7/10

N2 - ABCA3 is proposed to function as a lung surfactant lipid transporter. Here we report ABCA3-dependent lipid uptake into intracellular vesicles in lung adenocarcinoma A549 cells. A549 cells stably expressing GFP-tagged wild-type ABCA3 (A549/ABCA3WT) had larger LAMP3-positive vesicles than their parental cells as well as A549 cells expressing a Walker A motif mutant (A549/ABCA3N568D). The choline-phospholipids level in A549/ABCA3WT was increased 1.25-fold compared to that in A549 and A549/ABCA3N568D cells, while the cholesterol levels were similar. Sucrose gradient fractionation analysis in A549/ABCA3WT cells revealed that choline-phospholipids were enriched in low-density and nile red-positive vesicles. Electronmicroscopic analysis showed multilamellar vesicles in A549/ABCA3WT cells. These results indicate that ABCA3 mediates ATP-dependent choline-phospholipids uptake into intracellular vesicles.

AB - ABCA3 is proposed to function as a lung surfactant lipid transporter. Here we report ABCA3-dependent lipid uptake into intracellular vesicles in lung adenocarcinoma A549 cells. A549 cells stably expressing GFP-tagged wild-type ABCA3 (A549/ABCA3WT) had larger LAMP3-positive vesicles than their parental cells as well as A549 cells expressing a Walker A motif mutant (A549/ABCA3N568D). The choline-phospholipids level in A549/ABCA3WT was increased 1.25-fold compared to that in A549 and A549/ABCA3N568D cells, while the cholesterol levels were similar. Sucrose gradient fractionation analysis in A549/ABCA3WT cells revealed that choline-phospholipids were enriched in low-density and nile red-positive vesicles. Electronmicroscopic analysis showed multilamellar vesicles in A549/ABCA3WT cells. These results indicate that ABCA3 mediates ATP-dependent choline-phospholipids uptake into intracellular vesicles.

KW - Alveolar type II cell

KW - ATP-binding cassette transporter

KW - Choline-phospholipids

KW - Lamellar body

KW - Lipid transport

KW - Surfactant

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ER -

ABCA3-mediated choline-phospholipids uptake into intracellular vesicles in A549 cells

Abstract

Keywords

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