TY - JOUR
T1 - Absolute quantitation of myocardial blood flow with 201Tl and dynamic SPECT in canine
T2 - Optimisation and validation of kinetic modelling
AU - Iida, Hidehiro
AU - Eberl, Stefan
AU - Kim, Kyeong Min
AU - Tamura, Yoshikazu
AU - Ono, Yukihiko
AU - Nakazawa, Mayumi
AU - Sohlberg, Antti
AU - Zeniya, Tsutomu
AU - Hayashi, Takuya
AU - Watabe, Hiroshi
N1 - Funding Information:
Acknowledgement This study was supported by the Budget for Nuclear Research of the Ministry of Education, Culture, Sports, and Technology (MEXT), Japan; a grant from the Cooperative Link of Unique Science and Technology for Economy Revitalization promoted by the Ministry of Education, Culture, Sports and Technology, Japan and a grant for translational research from the Ministry of Health, Labour and Welfare (MHLW), Japan. We would like to thank Nihon Medi-Physics, Hyogo, Japan for providing the 201Tl samples and also Mr. Yoshihide Takatani for his invaluable suggestion on the study design.
PY - 2008/5
Y1 - 2008/5
N2 - Purpose: 201Tl has been extensively used for myocardial perfusion and viability assessment. Unlike 99mTc-labelled agents, such as 99mTc-sestamibi and 99mTc-tetrofosmine, the regional concentration of 201Tl varies with time. This study is intended to validate a kinetic modelling approach for in vivo quantitative estimation of regional myocardial blood flow (MBF) and volume of distribution of 201Tl using dynamic SPECT. Methods: Dynamic SPECT was carried out on 20 normal canines after the intravenous administration of 201Tl using a commercial SPECT system. Seven animals were studied at rest, nine during adenosine infusion, and four after beta-blocker administration. Quantitative images were reconstructed with a previously validated technique, employing OS-EM with attenuation-correction, and transmission-dependent convolution subtraction scatter correction. Measured regional time-activity curves in myocardial segments were fitted to two- and three-compartment models. Regional MBF was defined as the influx rate constant (K 1) with corrections for the partial volume effect, haematocrit and limited first-pass extraction fraction, and was compared with that determined from radio-labelled microspheres experiments. Results: Regional time-activity curves responded well to pharmacological stress. Quantitative MBF values were higher with adenosine and decreased after beta-blocker compared to a resting condition. MBFs obtained with SPECT (MBFSPECT) correlated well with the MBF values obtained by the radio-labelled microspheres (MBFMS) (MBFSPECT=-0.067+1. 042×MBFMS, p<0.001). The three-compartment model provided better fit than the two-compartment model, but the difference in MBF values between the two methods was small and could be accounted for with a simple linear regression. Conclusion: Absolute quantitation of regional MBF, for a wide physiological flow range, appears to be feasible using 201Tl and dynamic SPECT.
AB - Purpose: 201Tl has been extensively used for myocardial perfusion and viability assessment. Unlike 99mTc-labelled agents, such as 99mTc-sestamibi and 99mTc-tetrofosmine, the regional concentration of 201Tl varies with time. This study is intended to validate a kinetic modelling approach for in vivo quantitative estimation of regional myocardial blood flow (MBF) and volume of distribution of 201Tl using dynamic SPECT. Methods: Dynamic SPECT was carried out on 20 normal canines after the intravenous administration of 201Tl using a commercial SPECT system. Seven animals were studied at rest, nine during adenosine infusion, and four after beta-blocker administration. Quantitative images were reconstructed with a previously validated technique, employing OS-EM with attenuation-correction, and transmission-dependent convolution subtraction scatter correction. Measured regional time-activity curves in myocardial segments were fitted to two- and three-compartment models. Regional MBF was defined as the influx rate constant (K 1) with corrections for the partial volume effect, haematocrit and limited first-pass extraction fraction, and was compared with that determined from radio-labelled microspheres experiments. Results: Regional time-activity curves responded well to pharmacological stress. Quantitative MBF values were higher with adenosine and decreased after beta-blocker compared to a resting condition. MBFs obtained with SPECT (MBFSPECT) correlated well with the MBF values obtained by the radio-labelled microspheres (MBFMS) (MBFSPECT=-0.067+1. 042×MBFMS, p<0.001). The three-compartment model provided better fit than the two-compartment model, but the difference in MBF values between the two methods was small and could be accounted for with a simple linear regression. Conclusion: Absolute quantitation of regional MBF, for a wide physiological flow range, appears to be feasible using 201Tl and dynamic SPECT.
KW - Compartment model
KW - Dynamic SPECT
KW - Myocardial blood flow
KW - Quantitation
KW - Thallium-201
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U2 - 10.1007/s00259-007-0654-4
DO - 10.1007/s00259-007-0654-4
M3 - Article
C2 - 18202845
AN - SCOPUS:42249102087
SN - 1619-7070
VL - 35
SP - 896
EP - 905
JO - European Journal of Nuclear Medicine and Molecular Imaging
JF - European Journal of Nuclear Medicine and Molecular Imaging
IS - 5
ER -