Accumulation of chloroplast DNA sequences on the Y chromosome of Silene latifolia

Eduard Kejnovsky; Zdenek Kubat; Roman Hobza; Martina Lengerova; Shusei Sato; Satoshi Tabata; Kiichi Fukui; Sachihiro Matsunaga; Boris Vyskot

doi:10.1007/s10709-005-5701-0

Accumulation of chloroplast DNA sequences on the Y chromosome of Silene latifolia

Eduard Kejnovsky, Zdenek Kubat, Roman Hobza, Martina Lengerova, Shusei Sato, Satoshi Tabata, Kiichi Fukui, Sachihiro Matsunaga, Boris Vyskot

LIF - Ecological Developmental Adaptability Life Sciences

Research output: Contribution to journal › Article › peer-review

54 Citations (Scopus)

Abstract

Silene latifolia is a model dioecious plant with heteromorphic sex chromosomes. The Y chromosome is the largest in this species. Theoretical models propose an accumulation of repetitive DNA sequences in non-recombining parts of the Y chromosome. In this study, we isolated a BAC7H5 clone preferentially hybridizing to the Y chromosome of S. latifolia. Sequence analysis revealed that this BAC7H5 contains part of the chloroplast genome, indicating that these chloroplast sequences have accumulated on the Y chromosome and also may contribute to its large size. We constructed Y chromosome- and X chromosome-specific libraries and screened them to find Y- and/or X-linked copies of chloroplast sequences. Sequence analysis revealed higher divergence of a non-genic region of the chloroplast sequences located on the Y chromosome while genic regions tested showed only very low (max 0.9%) divergence from their chloroplast homologues.

Original language	English
Pages (from-to)	167-175
Number of pages	9
Journal	Genetica
Volume	128
Issue number	1-3
DOIs	https://doi.org/10.1007/s10709-005-5701-0
Publication status	Published - 2006 Sept

Keywords

Accumulation
Chloroplast DNA
Silene latifolia
Y chromosome

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10.1007/s10709-005-5701-0

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title = "Accumulation of chloroplast DNA sequences on the Y chromosome of Silene latifolia",

abstract = "Silene latifolia is a model dioecious plant with heteromorphic sex chromosomes. The Y chromosome is the largest in this species. Theoretical models propose an accumulation of repetitive DNA sequences in non-recombining parts of the Y chromosome. In this study, we isolated a BAC7H5 clone preferentially hybridizing to the Y chromosome of S. latifolia. Sequence analysis revealed that this BAC7H5 contains part of the chloroplast genome, indicating that these chloroplast sequences have accumulated on the Y chromosome and also may contribute to its large size. We constructed Y chromosome- and X chromosome-specific libraries and screened them to find Y- and/or X-linked copies of chloroplast sequences. Sequence analysis revealed higher divergence of a non-genic region of the chloroplast sequences located on the Y chromosome while genic regions tested showed only very low (max 0.9%) divergence from their chloroplast homologues.",

keywords = "Accumulation, Chloroplast DNA, Silene latifolia, Y chromosome",

author = "Eduard Kejnovsky and Zdenek Kubat and Roman Hobza and Martina Lengerova and Shusei Sato and Satoshi Tabata and Kiichi Fukui and Sachihiro Matsunaga and Boris Vyskot",

note = "Funding Information: This work was supported by the Grant Agency of the Czech Republic (grant nos. 204/05/2097, and 204/05/H505), by the institutional grant A50040507, a Grant-in-Aid of Scientific Research (grant no. 16770049) from the Ministry of Culture, Sports, Science and Technology of Japan, and the Kazusa DNA Research Institute Foundation. We thank Drs Jan Bartos and Jan Safar for printing the membranes, and Julia M. Svoboda, UC Davis, for critical reading of the manuscript.",

year = "2006",

month = sep,

doi = "10.1007/s10709-005-5701-0",

language = "English",

volume = "128",

pages = "167--175",

journal = "Genetica",

issn = "0016-6707",

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number = "1-3",

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T1 - Accumulation of chloroplast DNA sequences on the Y chromosome of Silene latifolia

AU - Kejnovsky, Eduard

AU - Kubat, Zdenek

AU - Hobza, Roman

AU - Lengerova, Martina

AU - Sato, Shusei

AU - Tabata, Satoshi

AU - Fukui, Kiichi

AU - Matsunaga, Sachihiro

AU - Vyskot, Boris

N1 - Funding Information: This work was supported by the Grant Agency of the Czech Republic (grant nos. 204/05/2097, and 204/05/H505), by the institutional grant A50040507, a Grant-in-Aid of Scientific Research (grant no. 16770049) from the Ministry of Culture, Sports, Science and Technology of Japan, and the Kazusa DNA Research Institute Foundation. We thank Drs Jan Bartos and Jan Safar for printing the membranes, and Julia M. Svoboda, UC Davis, for critical reading of the manuscript.

PY - 2006/9

Y1 - 2006/9

N2 - Silene latifolia is a model dioecious plant with heteromorphic sex chromosomes. The Y chromosome is the largest in this species. Theoretical models propose an accumulation of repetitive DNA sequences in non-recombining parts of the Y chromosome. In this study, we isolated a BAC7H5 clone preferentially hybridizing to the Y chromosome of S. latifolia. Sequence analysis revealed that this BAC7H5 contains part of the chloroplast genome, indicating that these chloroplast sequences have accumulated on the Y chromosome and also may contribute to its large size. We constructed Y chromosome- and X chromosome-specific libraries and screened them to find Y- and/or X-linked copies of chloroplast sequences. Sequence analysis revealed higher divergence of a non-genic region of the chloroplast sequences located on the Y chromosome while genic regions tested showed only very low (max 0.9%) divergence from their chloroplast homologues.

AB - Silene latifolia is a model dioecious plant with heteromorphic sex chromosomes. The Y chromosome is the largest in this species. Theoretical models propose an accumulation of repetitive DNA sequences in non-recombining parts of the Y chromosome. In this study, we isolated a BAC7H5 clone preferentially hybridizing to the Y chromosome of S. latifolia. Sequence analysis revealed that this BAC7H5 contains part of the chloroplast genome, indicating that these chloroplast sequences have accumulated on the Y chromosome and also may contribute to its large size. We constructed Y chromosome- and X chromosome-specific libraries and screened them to find Y- and/or X-linked copies of chloroplast sequences. Sequence analysis revealed higher divergence of a non-genic region of the chloroplast sequences located on the Y chromosome while genic regions tested showed only very low (max 0.9%) divergence from their chloroplast homologues.

KW - Accumulation

KW - Chloroplast DNA

KW - Silene latifolia

KW - Y chromosome

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Accumulation of chloroplast DNA sequences on the Y chromosome of Silene latifolia

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Keywords

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