Accumulation of plasma cells in atherosclerotic lesions of Watanabe heritable hyperlipidemic rabbits

Yoshiaki Sohma; Hironobu Sasano; Ryoko Shiga; Shigeru Saeki; Takashi Suzuki; Hiroshi Nagura; Masato Nose; Tokuo Yamamoto

doi:10.1073/pnas.92.11.4937

Accumulation of plasma cells in atherosclerotic lesions of Watanabe heritable hyperlipidemic rabbits

Yoshiaki Sohma, Hironobu Sasano, Ryoko Shiga, Shigeru Saeki, Takashi Suzuki, Hiroshi Nagura, Masato Nose, Tokuo Yamamoto

MED - Medical Sciences

Research output: Contribution to journal › Article › peer-review

36 Citations (Scopus)

Abstract

By screening a cDNA library constructed from aortic total RNA derived ham Watanabe heritable hyperlipidemic (WHHL) rabbits by differential hybridization, we have obtained a cDNA encoding the κ light chain of immunoglobulin. Northern blot analysis of total RNA prepared from aortas of WHHL and normal rabbits of various ages revealed that this light-chain mRNA accumulates gradually with age in aortas in WHHL rabbits. Northern blotting and in situ hybridization with an antisense oligonucleotide specific to rabbit immunoglobulin γ heavy-chain mRNA also detected accumulation of this heavy-chain mRNA in advanced lesions of WHHL rabbit aortas. Moreover, immunohistochemical and electron microscopic analyses demonstrated the presence of plasma cells in the atherosclerotic lesions.

Original language	English
Pages (from-to)	4937-4941
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	92
Issue number	11
DOIs	https://doi.org/10.1073/pnas.92.11.4937
Publication status	Published - 1995 May 23

Keywords

IgG
low density lipoprotein receptor deficient

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10.1073/pnas.92.11.4937

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abstract = "By screening a cDNA library constructed from aortic total RNA derived ham Watanabe heritable hyperlipidemic (WHHL) rabbits by differential hybridization, we have obtained a cDNA encoding the κ light chain of immunoglobulin. Northern blot analysis of total RNA prepared from aortas of WHHL and normal rabbits of various ages revealed that this light-chain mRNA accumulates gradually with age in aortas in WHHL rabbits. Northern blotting and in situ hybridization with an antisense oligonucleotide specific to rabbit immunoglobulin γ heavy-chain mRNA also detected accumulation of this heavy-chain mRNA in advanced lesions of WHHL rabbit aortas. Moreover, immunohistochemical and electron microscopic analyses demonstrated the presence of plasma cells in the atherosclerotic lesions.",

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AU - Sohma, Yoshiaki

AU - Sasano, Hironobu

AU - Shiga, Ryoko

AU - Saeki, Shigeru

AU - Suzuki, Takashi

AU - Nagura, Hiroshi

AU - Nose, Masato

AU - Yamamoto, Tokuo

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AB - By screening a cDNA library constructed from aortic total RNA derived ham Watanabe heritable hyperlipidemic (WHHL) rabbits by differential hybridization, we have obtained a cDNA encoding the κ light chain of immunoglobulin. Northern blot analysis of total RNA prepared from aortas of WHHL and normal rabbits of various ages revealed that this light-chain mRNA accumulates gradually with age in aortas in WHHL rabbits. Northern blotting and in situ hybridization with an antisense oligonucleotide specific to rabbit immunoglobulin γ heavy-chain mRNA also detected accumulation of this heavy-chain mRNA in advanced lesions of WHHL rabbit aortas. Moreover, immunohistochemical and electron microscopic analyses demonstrated the presence of plasma cells in the atherosclerotic lesions.

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Accumulation of plasma cells in atherosclerotic lesions of Watanabe heritable hyperlipidemic rabbits

Abstract

Keywords

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