TY - JOUR
T1 - Actin polymerization is activated by terahertz irradiation
AU - Yamazaki, Shota
AU - Harata, Masahiko
AU - Idehara, Toshitaka
AU - Konagaya, Keiji
AU - Yokoyama, Ginji
AU - Hoshina, Hiromichi
AU - Ogawa, Yuichi
N1 - Funding Information:
We would like to thank Dr. Isamu Ogawa for excellent technical assistance and advice and Mr. Yuto Takahashi for assistance with experiments. This work was supported by Japan Society for the Promotion of Science (JSPS) KAKENHI Grant Numbers JP25116009, JP15H04625, JP15K14706, JP16H05010, JP17H07365 and JP17J00302, and also by the JSPS Core-to-Core Program (Advanced Research Networks) entitled “Establishment of international agricultural immunology research-core for a quantum improvement in food safety”. Development of a high power sub-THz radiation source (Gyrotron FU CW GOIII) was partially supported by Grants in Aids from JSPS, SENTAN JST Cooperative Research Program of Institute for Protein Research, and NMR Platform MEXT, Japan.
Publisher Copyright:
© 2018 The Author(s).
PY - 2018/12/1
Y1 - 2018/12/1
N2 - Polymerization of monomeric actin into filaments has pivotal roles in cell motility, growth, differentiation, and gene expression. Therefore, techniques of manipulating actin polymerization, including actin-binding chemicals, have been developed for understanding and regulating multiple biological functions. Here, we demonstrate that irradiation with terahertz (THz) waves is a novel method of modulating actin polymerization. When actin polymerization reaction is performed under irradiation with 0.46 THz waves generated by a Gyrotron, actin polymerization was observed to be activated by monitoring the fluorescence of pyrene actin fluorophores. We also observed the number of actin filaments under a fluorescence microscope using the polymerized actin probe SiR-actin. The number of actin filaments was increased by 3.5-fold after THz irradiation for 20 min. When the THz irradiation was applied to a steady-state actin solution, in which elongation and depolymerization of actin filaments were equilibrated, increased actin polymerization was observed, suggesting that the THz irradiation activates actin polymerization, at least in the elongation process. These results suggest that THz waves could be applied for manipulating biomolecules and cells.
AB - Polymerization of monomeric actin into filaments has pivotal roles in cell motility, growth, differentiation, and gene expression. Therefore, techniques of manipulating actin polymerization, including actin-binding chemicals, have been developed for understanding and regulating multiple biological functions. Here, we demonstrate that irradiation with terahertz (THz) waves is a novel method of modulating actin polymerization. When actin polymerization reaction is performed under irradiation with 0.46 THz waves generated by a Gyrotron, actin polymerization was observed to be activated by monitoring the fluorescence of pyrene actin fluorophores. We also observed the number of actin filaments under a fluorescence microscope using the polymerized actin probe SiR-actin. The number of actin filaments was increased by 3.5-fold after THz irradiation for 20 min. When the THz irradiation was applied to a steady-state actin solution, in which elongation and depolymerization of actin filaments were equilibrated, increased actin polymerization was observed, suggesting that the THz irradiation activates actin polymerization, at least in the elongation process. These results suggest that THz waves could be applied for manipulating biomolecules and cells.
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U2 - 10.1038/s41598-018-28245-9
DO - 10.1038/s41598-018-28245-9
M3 - Article
C2 - 29968762
AN - SCOPUS:85049515819
SN - 2045-2322
VL - 8
JO - Scientific Reports
JF - Scientific Reports
IS - 1
M1 - 9990
ER -