TY - JOUR
T1 - Actin-related protein Arp4 functions in kinetochore assembly
AU - Ogiwara, Hideaki
AU - Ui, Ayako
AU - Kawashima, Satoshi
AU - Kugou, Kazuto
AU - Onoda, Fumitoshi
AU - Iwahashi, Hitoshi
AU - Harata, Masahiko
AU - Ohta, Kunihiro
AU - Enomoto, Takemi
AU - Seki, Masayuki
N1 - Funding Information:
We thank U. Wintersberger for plasmids used in this study. We thank all members of the Enomoto lab for their support. This work was supported by Grants-in-Aid for Scientific Research on Priority Areas from The Ministry of Education, Science, Sports and Culture of Japan, and by Health Sciences Research Grants from the Ministry of Health and Welfare of Japan. Funding to pay the Open Access publication charges for this article was provided by Grants-in-Aid for Scientific Research on Priority Areas from The Ministry of Education, Science, Sports and Culture of Japan.
PY - 2007/5
Y1 - 2007/5
N2 - The actin-related proteins (Arps) comprise a conserved protein family. Arp4p is found in large multisubunits of the INO80 and SWR1 chromatin remodeling complexes and in the NuA4 histone acetyltransferase complex. Here we show that arp4 (arp4S23A/D159A) temperature-sensitive cells are defective in G2/M phase function. arp4 mutants are sensitive to the microtubule depolymerizing agent benomyl and arrest at G2/M phase at restrictive temperature. Arp4p is associated with centromeric and telomeric regions throughout cell cycle. Ino80p, Esa1p and Swr1p, components of the INO80, NuA4 and SWR1 complexes, respectively, also associate with centromeres. The association of many kinetochore components including Cse4p, a component of the centromere nucleosome, Mtw1p and Ctf3p is partially impaired in arp4 cells, suggesting that the G2/M arrest of arp4 mutant cells is due to a defect in formation of the chromosomal segregation apparatus.
AB - The actin-related proteins (Arps) comprise a conserved protein family. Arp4p is found in large multisubunits of the INO80 and SWR1 chromatin remodeling complexes and in the NuA4 histone acetyltransferase complex. Here we show that arp4 (arp4S23A/D159A) temperature-sensitive cells are defective in G2/M phase function. arp4 mutants are sensitive to the microtubule depolymerizing agent benomyl and arrest at G2/M phase at restrictive temperature. Arp4p is associated with centromeric and telomeric regions throughout cell cycle. Ino80p, Esa1p and Swr1p, components of the INO80, NuA4 and SWR1 complexes, respectively, also associate with centromeres. The association of many kinetochore components including Cse4p, a component of the centromere nucleosome, Mtw1p and Ctf3p is partially impaired in arp4 cells, suggesting that the G2/M arrest of arp4 mutant cells is due to a defect in formation of the chromosomal segregation apparatus.
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U2 - 10.1093/nar/gkm161
DO - 10.1093/nar/gkm161
M3 - Article
C2 - 17452364
AN - SCOPUS:34250640741
SN - 0305-1048
VL - 35
SP - 3109
EP - 3117
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 9
ER -