Activation mechanism of c-Jun amino-terminal kinase in the course of endodermal differentiation of P19 embryonic carcinoma cells

Tada Aki Kudo; Yoshiyuki Sakamoto; Shinri Tamura; Takayasu Kobayashi

doi:10.1016/S0014-5793(03)00179-0

Activation mechanism of c-Jun amino-terminal kinase in the course of endodermal differentiation of P19 embryonic carcinoma cells

Tada Aki Kudo, Yoshiyuki Sakamoto, Shinri Tamura, Takayasu Kobayashi

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

c-Jun amino-terminal kinase (JNK) is known to be activated and play critical roles during neural and endodermal differentiation of P19 embryonic carcinoma cells. In this study we demonstrated that of the two upstream protein kinases of JNK, only MKK4 activity was substantially enhanced in the endodermally differentiating P19 cells. This enhanced activity of MKK4 stemmed from the increased expression of MKK4 and its activation by phosphorylation. Activated MKK4 and JNK were localized in both nucleus and cytoplasm of the differentiating cells, while they were localized only in the nucleus in the undifferentiated cells, suggesting multiple roles of JNK in the course of the endodermal differentiation of P19 cells.

Original language	English
Pages (from-to)	29-33
Number of pages	5
Journal	FEBS Letters
Volume	539
Issue number	1-3
DOIs	https://doi.org/10.1016/S0014-5793(03)00179-0
Publication status	Published - 2003 Mar 27

Keywords

Differentiation
Mitogen-activated protein kinase kinase 4
P19 embryonic carcinoma cell
c-Jun amino-terminal kinase

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(03)00179-0

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title = "Activation mechanism of c-Jun amino-terminal kinase in the course of endodermal differentiation of P19 embryonic carcinoma cells",

abstract = "c-Jun amino-terminal kinase (JNK) is known to be activated and play critical roles during neural and endodermal differentiation of P19 embryonic carcinoma cells. In this study we demonstrated that of the two upstream protein kinases of JNK, only MKK4 activity was substantially enhanced in the endodermally differentiating P19 cells. This enhanced activity of MKK4 stemmed from the increased expression of MKK4 and its activation by phosphorylation. Activated MKK4 and JNK were localized in both nucleus and cytoplasm of the differentiating cells, while they were localized only in the nucleus in the undifferentiated cells, suggesting multiple roles of JNK in the course of the endodermal differentiation of P19 cells.",

keywords = "Differentiation, Mitogen-activated protein kinase kinase 4, P19 embryonic carcinoma cell, c-Jun amino-terminal kinase",

author = "Kudo, {Tada Aki} and Yoshiyuki Sakamoto and Shinri Tamura and Takayasu Kobayashi",

note = "Funding Information: This work was supported in part by a Grant-in-Aid for Scientific Research from Ministry of Education, Culture, Sports, Science and Technology of Japan (for S.T. and T.K.).",

year = "2003",

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doi = "10.1016/S0014-5793(03)00179-0",

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T1 - Activation mechanism of c-Jun amino-terminal kinase in the course of endodermal differentiation of P19 embryonic carcinoma cells

AU - Kudo, Tada Aki

AU - Sakamoto, Yoshiyuki

AU - Tamura, Shinri

AU - Kobayashi, Takayasu

N1 - Funding Information: This work was supported in part by a Grant-in-Aid for Scientific Research from Ministry of Education, Culture, Sports, Science and Technology of Japan (for S.T. and T.K.).

PY - 2003/3/27

Y1 - 2003/3/27

N2 - c-Jun amino-terminal kinase (JNK) is known to be activated and play critical roles during neural and endodermal differentiation of P19 embryonic carcinoma cells. In this study we demonstrated that of the two upstream protein kinases of JNK, only MKK4 activity was substantially enhanced in the endodermally differentiating P19 cells. This enhanced activity of MKK4 stemmed from the increased expression of MKK4 and its activation by phosphorylation. Activated MKK4 and JNK were localized in both nucleus and cytoplasm of the differentiating cells, while they were localized only in the nucleus in the undifferentiated cells, suggesting multiple roles of JNK in the course of the endodermal differentiation of P19 cells.

AB - c-Jun amino-terminal kinase (JNK) is known to be activated and play critical roles during neural and endodermal differentiation of P19 embryonic carcinoma cells. In this study we demonstrated that of the two upstream protein kinases of JNK, only MKK4 activity was substantially enhanced in the endodermally differentiating P19 cells. This enhanced activity of MKK4 stemmed from the increased expression of MKK4 and its activation by phosphorylation. Activated MKK4 and JNK were localized in both nucleus and cytoplasm of the differentiating cells, while they were localized only in the nucleus in the undifferentiated cells, suggesting multiple roles of JNK in the course of the endodermal differentiation of P19 cells.

KW - Differentiation

KW - Mitogen-activated protein kinase kinase 4

KW - P19 embryonic carcinoma cell

KW - c-Jun amino-terminal kinase

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JF - FEBS Letters

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ER -

Activation mechanism of c-Jun amino-terminal kinase in the course of endodermal differentiation of P19 embryonic carcinoma cells

Abstract

Keywords

ASJC Scopus subject areas

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